| Screening of cellulases for biofuel production: online monitoring of the enzymatic hydrolysis of insoluble cellulose using high-throughput scattered light detection. | |
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MedLine Citation:
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PMID: 21184439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new prospective cellulase assay simultaneously combining high-throughput, online analysis and insoluble cellulosic substrates is described. The hydrolysis of three different insoluble cellulosic substrates, catalysed by a commercial cellulase preparation from Trichoderma reesei (Celluclast), was monitored using the BioLector - allowing online monitoring of scattered light intensities in a continuously shaken microtiter plate. Cellulase activities could be quantitatively assayed using the BioLector. At low cellulase/cellulose ratios, the Michaelis-Menten parameters of the cellulase mixture were mainly affected by the crystallinity index of the cellulose. Here, the apparent maximum cellulase activities inversely correlated with the crystallinity index of the cellulose. At high cellulase/cellulose ratios the particle size of the cellulose, defining the external surface area accessible to the cellulases, was the key determining factor for cellulase activity. The developed technique was also successfully applied to evaluate the pH optimum of cellulases. Moreover, the non-hydrolytic deagglomeration of cellulose particles was investigated, for the first time, using high-throughput scattered light detection. In conclusion, this cellulase assay ideally links high-throughput, online analysis and realistic insoluble cellulosic substrates in one simple system. It will considerably simplify and accelerate fundamental research on cellulase screening. |
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Authors:
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Gernot Jäger; Helene Wulfhorst; Erik U Zeithammel; Efthimia Elinidou; Antje C Spiess; Jochen Büchs |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology journal Volume: 6 ISSN: 1860-7314 ISO Abbreviation: Biotechnol J Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-12 Completed Date: 2011-04-28 Revised Date: 2011-12-15 |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: Germany |
Other Details:
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Languages: eng Pagination: 74-85 Citation Subset: IM |
Affiliation:
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AVT-Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biofuels* Biotechnology Cellulase / metabolism* Cellulose / metabolism* Hydrogen-Ion Concentration Hydrolysis Trichoderma / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Biofuels; 9004-34-6/Cellulose; EC 3.2.1.4/Cellulase |
| Comments/Corrections | |
Erratum In:
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Biotechnol J. 2011 Nov;6(11):1419 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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