Document Detail


Screening of budesonide nanoformulations for treatment of inflammatory bowel disease in an inflamed 3D cell-culture model.
MedLine Citation:
PMID:  22847255     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Drug formulation screenings for treatment of inflammatory bowel disease (IBD) are mostly conducted in chemically induced rodent models that represent acute injury-caused inflammation instead of a chronic condition. To accurately screen drug formulations for chronic IBD, a relevant model that mimics the chronic condition in vitro is urgently needed. In an effort to reduce and potentially replace this scientifically and ethically questionable animal testing for IBD drugs, our laboratory has developed an in vitro model for the inflamed intestinal mucosa observed in chronic IBD, which allows high-throughput screening of anti-inflammatory drugs and their formulations. The in vitro model consists of intestinal epithelial cells, human blood-derived macrophages, and dendritic cells that are stimulated by the inflammatory cytokine interleukin-1β. In this study, the model was utilized for evaluation of the efficacy and deposition of budesonide, an anti-inflammatory drug, in three different pharmaceutical formulations: (1) a free drug solution, (2) encapsulated into PLGA nanoparticles, and (3) encapsulated into liposomes. The in vitro model of the inflamed intestinal mucosa demonstrated its ability to differentiate therapeutic efficacy among the formulations while maintaining the convenience of conventional in vitro studies and adequately representing the complex pathophysiological changes observed in vivo.
Authors:
Fransisca Leonard; Hussain Ali; Eva-Maria Collnot; Bart J Crielaard; Twan Lammers; Gert Storm; Claus-Michael Lehr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  ALTEX     Volume:  29     ISSN:  1868-596X     ISO Abbreviation:  ALTEX     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953980     Medline TA:  ALTEX     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  275-85     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany.
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