| Screening for autologous blood transfusions. | |
| | |
MedLine Citation:
|
PMID: 19199200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The ratio between the amount of hemoglobin in the mature erythrocyte population and the reticulocytes (RBCHb:RetHb ratio) has previously been suggested as a marker to screen for EPO-abuse. We speculated that the reinfusion of blood would lead to a marked increase in this ratio, making it a valuable parameter in the screening for autologous blood doping. Three bags of blood (approximately 201+/-11 g of Hb) were withdrawn from 16 males and stored at either -80 degrees C (-80 T, n=8) or +4 degrees C (+4 T, n=8) and reinfused 10 weeks or 4 weeks later, respectively. Seven subjects served as controls. Different erythrocyte parameters were measured on a hematological analyzer serially throughout and during a 4 week wash-out period. By using RBCHb:RetHb ratio cut-off limits of 145.7 (1:100) ('suspicious') and 182.9 (1:1000) ('positive'), 35.4% (-80 T) and 19.6% (+4 T) of all samples obtained during a 4 week wash-out period were identified as 'suspicious', and 18.8% (-80 T) and 4.3% (+4 T) as 'positive'. In total, 7 out of 16 (43.8%) subjects had at least one sample exceeding 182.9. Compared to the currently used indirect parameters, the RBCHb:RetHb ratio is the best indicator of autologous blood doping after reinfusion, and the parameter could be used in a testing setting, once stability validation has been performed. |
| | |
Authors:
|
J Mørkeberg; B Belhage; M Ashenden; A Bornø; K Sharpe; M H Dziegiel; R Damsgaard |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-06 |
Journal Detail:
|
Title: International journal of sports medicine Volume: 30 ISSN: 1439-3964 ISO Abbreviation: Int J Sports Med Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-03-26 Completed Date: 2009-06-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008349 Medline TA: Int J Sports Med Country: Germany |
Other Details:
|
Languages: eng Pagination: 285-92 Citation Subset: IM |
Affiliation:
|
Department of Anaesthesiology, Bispebjerg Hospital, Copenhagen NV, Denmark. jakobmoerkeberg@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Blood Preservation / methods Blood Transfusion, Autologous* Doping in Sports / methods* Erythrocytes / metabolism Hemoglobins / analysis*, metabolism Humans Male Reticulocytes / metabolism Substance Abuse Detection / methods* Temperature Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/Hemoglobins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Military diving training improved arterial compliance.
Next Document: Effects of differentiated music on cycling time trial.