| Screening of antifeedant activity in brain extracts led to the identification of sulfakinin as a satiety promoter in the German cockroach. Are arthropod sulfakinins homologous to vertebrate gastrins-cholecystokinins? | |
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MedLine Citation:
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PMID: 11722569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The feeding cycle of the adult female cockroach Blattella germanica parallels vitellogenesis. The study of the mechanisms that regulate this cycle led us to look for food-intake inhibitors in brain extracts. The antifeedant activity of brain extracts was tested in vivo by injecting the extract and measuring the carotenoids contained in the gut from carrot ingested after the treatment. By HPLC fractionation and tracking the biological activity with the carrot test, we isolated the sulfakinin EQFDDY(SO3H) GHMRFamide (Pea-SK). A synthetic version of the peptide inhibited food intake when injected at doses of 1 microg (50% inhibition) and 10 microg (60% inhibition). The sulfate group was required for food-intake inhibition. These biological and structural features are similar to those of the gastrin-cholecystokinin (gastrin-CCK) family of vertebrate peptides. However, heterologous feeding assays (human CCK-8 tested on B. germanica, and Pea-SK tested on the goldfish Carassius auratus) were negative. In spite of this, alignment and cluster analysis of these and other structurally similar peptide families suggest that sulfakinins and gastrin-CCKs are homologous, and that mechanisms of feeding regulation involving these regulatory peptides may have been conserved during evolution between insects and vertebrates. |
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Authors:
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J L Maestro; R Aguilar; N Pascual; M L Valero; M D Piulachs; D Andreu; I Navarro; X Bellés |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 268 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-27 Completed Date: 2001-12-31 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: Germany |
Other Details:
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Languages: eng Pagination: 5824-30 Citation Subset: IM |
Affiliation:
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Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Appetite / drug effects* Brain Chemistry* Cholecystokinin / chemistry* Chromatography, High Pressure Liquid Cluster Analysis Cockroaches / physiology* Feeding Behavior / drug effects* Female Gastrins / chemistry* Goldfish / physiology Mass Spectrometry Molecular Sequence Data Neuropeptides / chemistry, isolation & purification, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Gastrins; 0/Neuropeptides; 105520-56-7/leucosulfakinin; 9011-97-6/Cholecystokinin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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