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Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation.
MedLine Citation:
PMID:  24083697     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective: To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells. Method: After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs. Results: 70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results. Conclusion: Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.
Authors:
Chang-Xin Huang; Ying Zhu; Guang-Liang Duan; Ji-Fen Yao; Zhao-Yang Li; Da Li; Qing-Qing Wang
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Asian Pacific journal of cancer prevention : APJCP     Volume:  14     ISSN:  1513-7368     ISO Abbreviation:  Asian Pac. J. Cancer Prev.     Publication Date:  2013  
Date Detail:
Created Date:  2013-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101130625     Medline TA:  Asian Pac J Cancer Prev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  4533-4537     Citation Subset:  -    
Affiliation:
Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine, Hangzhou, China E-mail : hcx588@hotmail.com, hcx588@tom.com.
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