| Screening of bacterial isolates from polluted soils exhibiting catalase and peroxidase activity and diversity of their responses to oxidative stress. | |
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MedLine Citation:
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PMID: 20145932 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For the survival of individual isolates of gram-negative bacteria Pseudomonas putida, Achromobacter xylosoxidans, and the gram-positive bacterium Bacillus megaterium, in an environment polluted with crude oil products, the production of catalases exhibiting both catalase and dianisidine-peroxidase activity is important. Electrophoretic resolution of cell-free extracts of aerobically grown strains in Luria-Bertani medium during exponential phase revealed distinctive expression of catalatic and peroxidatic activities detected with 3,3'-diaminobenzidine tetrahydrochloride. A considerable diversity in microbial catalase and peroxidase responses to 20 or 40 mM H(2)O(2) stress, resulted from hydroperoxidase's variant of original isolates, indicating an environmental selective pressure. However, catalase was important for the adaptation of cultures to high concentration of 60 mM H(2)O(2). Appreciable differences in the sensitivity to toxic effect of H(2)O(2) (20 or 40 mM) treatment between individual isolates and their adapted variants during growth were observed until the middle of exponential phase, but they were insignificant at the entry to stationary phase. Isolates also exhibited a considerable diversity in catalases responses to phenolic contaminants 1 and 2 mM o- or p-phenylenediamine. Catalase activity of bacterium P. putida was visibly stimulated only by p-phenylenediamine and not by its positional isomer o-PDA. This study contributes to a better understanding of the role catalases play in bacterial responses to a polluted environment. |
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Authors:
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Mária Bucková; Jana Godocíková; Marcel Zámocký; Bystrík Polek |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
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Title: Current microbiology Volume: 61 ISSN: 1432-0991 ISO Abbreviation: Curr. Microbiol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-20 Completed Date: 2011-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 241-7 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551 Bratislava, Slovakia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Achromobacter denitrificans
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growth & development,
metabolism* Bacillus megaterium / growth & development, metabolism* Catalase / metabolism* Electrophoresis, Polyacrylamide Gel Environmental Pollution Hydrogen Peroxide / metabolism Oxidative Stress* Peroxidase / metabolism* Petroleum Phenylenediamines / metabolism Pseudomonas putida / growth & development, metabolism* Reactive Oxygen Species / metabolism Soil Microbiology* Soil Pollutants / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Petroleum; 0/Phenylenediamines; 0/Reactive Oxygen Species; 0/Soil Pollutants; 106-50-3/4-phenylenediamine; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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