Document Detail


Screen-printed amperometric biosensors for the rapid measurement of L- and D-amino acids.
MedLine Citation:
PMID:  10736871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinized carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilized enzyme. The devices responded to all 20 common L-amino acids and all of the D-amino acids examined, the exceptions being L- and D-proline. Linear response profiles were observed for L-leucine, L-glycine and L-phenylalanine with limits of detection of 0.47, 0.15 and 0.20 mM respectively. The devices were reproducible and exhibited stability over a 56 d test period. The biosensor compares favourably with a standard photometric amino acid test and was used to monitor milk ageing effects. The assay is cheap, simple to perform and rapid, requiring only buffer-electrolyte and a small sample volume.
Authors:
P Sarkar; I E Tothill; S J Setford; A P Turner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Analyst     Volume:  124     ISSN:  0003-2654     ISO Abbreviation:  Analyst     Publication Date:  1999 Jun 
Date Detail:
Created Date:  2000-04-03     Completed Date:  2000-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  865-70     Citation Subset:  IM    
Affiliation:
Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
Biological Markers / analysis
Biosensing Techniques*
Cattle
Food Microbiology*
Isomerism
Milk / microbiology*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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