| Screen-printed amperometric biosensors for the rapid measurement of L- and D-amino acids. | |
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MedLine Citation:
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PMID: 10736871 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinized carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilized enzyme. The devices responded to all 20 common L-amino acids and all of the D-amino acids examined, the exceptions being L- and D-proline. Linear response profiles were observed for L-leucine, L-glycine and L-phenylalanine with limits of detection of 0.47, 0.15 and 0.20 mM respectively. The devices were reproducible and exhibited stability over a 56 d test period. The biosensor compares favourably with a standard photometric amino acid test and was used to monitor milk ageing effects. The assay is cheap, simple to perform and rapid, requiring only buffer-electrolyte and a small sample volume. |
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Authors:
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P Sarkar; I E Tothill; S J Setford; A P Turner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Analyst Volume: 124 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 1999 Jun |
Date Detail:
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Created Date: 2000-04-03 Completed Date: 2000-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 865-70 Citation Subset: IM |
Affiliation:
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Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Animals Biological Markers / analysis Biosensing Techniques* Cattle Food Microbiology* Isomerism Milk / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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