Document Detail

Scrapie strain infection in vitro induces changes in neuronal cells.
MedLine Citation:
PMID:  7999309     Owner:  NLM     Status:  MEDLINE    
PC12 cells, in the presence of nerve growth factor (NGF), support replication of the mouse-derived scrapie strains 139A and ME7, with the former yielding 100-1000-fold higher levels of infectivity. Infectivity remained cell-associated and cells did not show any gross morphological alterations, although changes were observed by electron microscopy in the form of an increased number of lipid droplets in 139A-infected cultures. Analysis of phospholipid metabolism in 139A infected cells indicated that scrapie replication did not change the inositol phosphate levels, but did stimulate phosphoinositide synthesis. Replication was not detected in PC12 cells infected with either the hamster-derived 263K or rat-derived 139R scrapie strains. Since scrapie-infected cultures did not exhibit cell death or any gross changes, any scrapie-induced effects would probably be manifested in nonvital cellular functions. When compared to controls, infection with the 139A scrapie strain resulted in decreased activity of the cholinergic pathway-related enzymes, as well as the GABA synthetic pathway; however, the adrenergic pathway was unaffected by scrapie infection. The effects of the 139A scrapie strain on the cholinergic system appeared to be dose-dependent and were first detected prior to the detection of scrapie agent replication in these cells. No neurotransmitter-related enzymatic changes were detected in 263K- or 139R-infected PC12 cells. The enzymatic changes observed in ME7-infected PC12 cells and in Chandler agent-infected mouse neuroblastoma cells suggest that the significant changes in neurotransmitter levels in cultures exhibiting low infectivity titers must involve factors other than, but not excluding, replication of the agent. The role of additional factors is also suggested in studies of protein kinase C activity in 139A- and 139R-infected PC12 cells. These studies emphasize the value of the PC12 cell model system in examining the scrapie strain-host cell interaction and, in addition, support the concept of variation among scrapie strains.
R Rubenstein; H Deng; R Race; W Ju; C Scalici; M Papini; A Rubenstein; R Kascsak; R Carp
Related Documents :
2167029 - Temporal appearance of structural and nonstructural bluetongue viral proteins in infect...
1064029 - An enzyme system for replication of duplex circular dna: the replicative form of phage ...
16188999 - Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early...
17506819 - An nsp4-dependant mechanism by which rotavirus impairs lactase enzymatic activity in br...
1604809 - Lack of direct correlation between p220 cleavage and the shut-off of host translation a...
11177539 - Manipulation of the cytoplasmic and transmembrane domains alters cell surface levels of...
22112559 - Transglutaminase-catalyzed preparation of human elastin-like polypeptide-based three-di...
21189159 - Rapid functional screening of effective sirnas against plk1 and its growth inhibitory e...
21344189 - Hydrogen peroxide is not involved in hrpn from erwinia amylovora-induced hypersensitive...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Molecular neurobiology     Volume:  8     ISSN:  0893-7648     ISO Abbreviation:  Mol. Neurobiol.     Publication Date:    1994 Apr-Jun
Date Detail:
Created Date:  1995-01-25     Completed Date:  1995-01-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8900963     Medline TA:  Mol Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  129-38     Citation Subset:  IM    
Department of Virology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, Staten Island, NY 10314.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acetylcholinesterase / metabolism
Brain / metabolism
Choline O-Acetyltransferase / metabolism
Nerve Growth Factors / pharmacology
Neurons / metabolism*
Neurotransmitter Agents / biosynthesis
PC12 Cells
PrPSc Proteins / metabolism*,  pathogenicity*
Scrapie / metabolism*
Tumor Cells, Cultured
Virus Replication
gamma-Aminobutyric Acid / biosynthesis
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors; 0/Neurotransmitter Agents; 0/PrPSc Proteins; 56-12-2/gamma-Aminobutyric Acid; EC O-Acetyltransferase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally norm...
Next Document:  The presence of HTLV-I proviral DNA in the central nervous system of patients with HTLV-I-associated...