Document Detail

Scopolamine Production in Suspension Cultures and Redifferentiated Roots of Hyoscyamus niger.
MedLine Citation:
PMID:  17404913     Owner:  NLM     Status:  In-Data-Review    
Adventitious roots were induced from 2-year-old, hyoscyamine rich suspension cultures of HYOSCYAMUS NIGER L. The cultured roots grew well in Linsmaier-Skoog medium containing 10 (-8)M 6-benzyladenine with 3% sucrose and produced much more scopolamine (0.12-0.30% dW) than hyoscyamine (0.04-0.08% dW). The alkaloids were detected in the medium as well as in the cells. Ten to twenty percent of the hyoscyamine added to the medium was converted to scopolamine within 24 days by the root cultures, but only 1 to 3% was converted by the suspension cultures. Scarcely any of the scopolamine added to the medium was converted to hyoscyamine by either culture.
T Hashimoto; Y Yamada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  47     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  2007-04-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  -    
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Kyoto, Japan.
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