Document Detail

Scintigraphic detection of neural-cell-derived small-cell lung cancer using glioma-specific antibody.
MedLine Citation:
PMID:  8126056     Owner:  NLM     Status:  MEDLINE    
Radiolabeled GA-17, a murine monoclonal antibody that reacts specifically with glioma cells, bound to a small-cell lung cancer (SCLC) cell line NCI-H69 derived from neural cells, both in vitro and in vivo. The affinity constant of GA-17 F (ab')2 fragment binding to NCI-H69 was 1.02 x 10(8)/M while that to the glioma cell line U87MG was 1.22 x 10(8)/M. Iodine-125-labeled GA-17 F(ab')2 fragments injected i.v. localized well in NCI-H69 cells xenografted in nude mice. The percentage of the injected dose per gram accumulated in the xenografted tumor was 6.87 +/- 1.34% g-1 (mean +/- SD, n = 5) 24 h after injection. On the other hand, control monoclonal F(ab')2 fragments accumulated in the xenografted tumor at 0.75 +/- 0.30% g-1. The tumor-to-blood ratio was 1.8 for NCI-H69, while that of control F(ab')2 was 0.60. In conclusion, the radiolabeled GA-17 F(ab')2 fragment is expected to be useful clinically to visualize the small-cell lung cancer and in radioimmunotherapy.
H Kobayashi; H Sakahara; M Hosono; M Shirato; S Kondo; S Miyatake; H Kikuchi; Y Namba; K Endo; J Konishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  120     ISSN:  0171-5216     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  1994  
Date Detail:
Created Date:  1994-04-12     Completed Date:  1994-04-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  259-62     Citation Subset:  IM    
Department of Nuclear Medicine, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Antibodies, Monoclonal / immunology*,  metabolism
Antibody Specificity
Carcinoma, Small Cell / immunology,  radionuclide imaging*
Glioma / immunology*
Immunoglobulin Fragments / immunology,  metabolism
Immunotoxins / diagnostic use*,  metabolism
Iodine Radioisotopes / diagnostic use
Lung Neoplasms / immunology,  radionuclide imaging*
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Tissue Distribution
Transplantation, Heterologous
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin Fragments; 0/Immunotoxins; 0/Iodine Radioisotopes

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