Document Detail


Schwann cells stimulated to proliferate in the absence of neurons retain full functional capability.
MedLine Citation:
PMID:  3760949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Schwann cells from neonatal rat sciatic nerve can be maintained and grown in culture in the absence of neurons. We are interested in substantially expanding such cultures for use in the study of Schwann cells, their growth responses, and their interactions with neurons. However, it was important to determine if expanded cell populations retained their distinguishing biological properties and their ability to differentiate when recombined with neurons. Therefore, we have compared the functional properties of extensively expanded populations of sciatic nerve Schwann cells to those of embryonic dorsal root ganglion (DRG) Schwann cells that had been briefly expanded in vitro in the continuous presence of ganglion neurons. Sciatic nerve Schwann cells were cultured and purified according to the methods of Brockes et al. (1979). A combination of crude glial growth factor and forskolin was found to act synergistically in providing maximal stimulation of Schwann cell proliferation. Sciatic nerve Schwann cells that were continuously expanded for at least 2 months were compared to Schwann cells derived from fetal dorsal root ganglia. The results indicate that the complement of secreted proteins from both cell populations, either in isolation or recombined with neurons, was essentially identical; both cell populations expressed the cell-surface antigens laminin and Ran 1 (217C antibody); after seeding onto DRG neurons, both cell populations associated with neuronal processes with the same time course; and under identical nutrient conditions, both cell populations were observed to exhibit a comparable capacity for myelination of DRG axons in vitro. Thus, methods used to establish primary cultures of rat sciatic nerve Schwann cells and to expand secondary cultures in vitro in the absence of neurons preserve basic Schwann cell functions.
Authors:
S Porter; M B Clark; L Glaser; R P Bunge
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  6     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-11-12     Completed Date:  1986-11-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3070-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Surface / analysis
Cell Division
Cells, Cultured
Mitogens / pharmacology
Myelin Sheath / physiology
Nerve Tissue Proteins / metabolism
Neurons / physiology
Schwann Cells / cytology,  immunology,  physiology*
Stimulation, Chemical
Grant Support
ID/Acronym/Agency:
NS07551/NS/NINDS NIH HHS; NS09923/NS/NINDS NIH HHS; NS19923/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Mitogens; 0/Nerve Tissue Proteins

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