| Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M. | |
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MedLine Citation:
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PMID: 1321159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immature Schwann cells of the rat sciatic nerve can differentiate into myelin-forming or non-myelin-forming cells. The factors that influence this divergent development are unknown but certain markers such as galactocerebroside distinguish the two cell populations at an early stage of Schwann cell differentiation. Because myelination requires extensive changes in cell morphology, we have investigated the composition and structure of the Schwann cell cytoskeleton at a time when these cells become committed to myelination. Here we show that Schwann cells express a cytoskeletal protein of M(r) 145 before diverging into the myelin-forming path, i.e., before they acquire cell-surface galactocerobroside. The p145 protein has the characteristics of an intermediate filament (IF) protein and immunoelectron microscopy shows that it colocalizes with vimentin, which suggests that these two proteins can coassemble into IFs. Elevated intracellular cAMP levels, which can mimic some of the early effects of axons on Schwann cell differentiation, induced p145 synthesis, therefore, we conclude that myelin-forming Schwann cells express this protein at a very early stage in their development. Immunological comparisons with other IF proteins revealed a close similarity between p145 and the neurofilament protein NF-M; the identification of p145 as NF-M was confirmed by isolating and sequencing a full-length clone from a Schwann cell cDNA library. These data demonstrate that Schwann cells remodel their IFs by expressing NF-M before acquiring the myelin-forming phenotype and that IF proteins of the neurofilament-type are not restricted to neurons in the vertebrate nervous system. |
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Authors:
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B M Kelly; C S Gillespie; D L Sherman; P J Brophy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 118 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-08-14 Completed Date: 1992-08-14 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 397-410 Citation Subset: IM |
Affiliation:
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Department of Biological and Molecular Sciences, University of Stirling, Scotland, UK. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M31165; M31166; M74319; M74320; M74321; M74322; M74323; M74324; X65191; Z12152 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Animals, Newborn Base Sequence Blotting, Western Bucladesine / pharmacology Cells, Cultured Colchicine / pharmacology Cyclic AMP / metabolism Cytoskeleton / drug effects, physiology*, ultrastructure DNA / genetics Fluorescent Antibody Technique Gene Library Microscopy, Immunoelectron Molecular Sequence Data Myelin Proteins / analysis, biosynthesis* Neurofilament Proteins / analysis, genetics* Phenotype Rats Schwann Cells / cytology, drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Myelin Proteins; 0/Neurofilament Proteins; 111365-29-8/neurofilament protein M; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 64-86-8/Colchicine; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol Journal ID (publisher-id): J. Cell Biol. ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 7 Year: 1992 Volume: 118 Issue: 2 First Page: 397 Last Page: 410 ID: 2290038 Publisher Id: 92332596 PubMed Id: 1321159 |
| Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M | |
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