| Schwann cell proliferation following lysolecithin-induced demyelination. | |
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MedLine Citation:
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PMID: 2391539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Schwann cell division, meticulously regulated throughout development, occurs at an extremely low level in normal adult nerves. Loss of the myelin sheath in disease results in active proliferation of Schwann cells. The dividing cells are usually thought to be the Schwann cells of the demylinated fibres and their daughters. In this study we asked if other populations of Schwann cells might also divide following focal monophasic demyelination, and if the proliferating Schwann cells would be found only in the foci of demyelination. [3H]thymidine incorporation was examined by autoradiography at intervals after topical application of lysolecithin (lysophosphatidyl choline) to rat sciatic nerves. The postlabelling intervals were set to identify premitotic cells, cells shortly after mitosis (perimitotic cells) and postmitotic cells, as well as to provide cumulative labelling over 3 days. The affected nerves had three distinct zones. The first was a zone of nearly complete demyelination immediately beneath the perineurium. The subjacent zone was normal morphologically except for numerous supernumerary Schwann cells, displacement of some Schwann cell perikarya, ultrastructural changes in a few myelinated fibres, and rare demyelinated and remyelinated fibres. The third zone, beneath the first two, was normal. In the focus of demyelination there were large numbers of Schwann cells in S phase on days 4 and 6. These cells included premyelinating Schwann cells that were contacting or ensheathing demyelinated axons or collateral axonal sprouts. The subjacent region also contained dividing Schwann cells, most of which were Schwann cells of unmyelinated Remak fibres. In addition, occasional Schwann cells of thickly myelinated fibres (fibres that had not previously undergone demyelination) were labelled by the premitotic schedule; most of these fibres had morphological abnormalities in the Schwann cell perikaryon or myelin sheath. In many, the perikaryon of the Schwann cell was beginning to separate from the rest of the Schwann cell cytoplasm and the myelin sheath. These changes suggested that these fibres were destined to undergo subsequent demyelination, a hypothesis supported by the absence of any normal myelinated fibres with labelled Schwann cell nuclei in nerves removed 1 week after labelling. Thus, this model provided no evidence for division by Schwann cells that continued to maintain myelin sheaths. Taken together, these results suggest that there is a 'surround' of Schwann cell proliferation around foci of demyelination; in this surround multiple populations of Schwann cells are recruited to proliferate, including Schwann cells of intact unmyelinated fibres. Structurally normal unmyelinated fibres appear to provide an unexpected source of new Schwann cells in nerve disease. |
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Authors:
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J W Griffin; E A Stocks; K Fahnestock; A Van Praagh; B D Trapp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurocytology Volume: 19 ISSN: 0300-4864 ISO Abbreviation: J. Neurocytol. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-10-04 Completed Date: 1990-10-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0364620 Medline TA: J Neurocytol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 367-84 Citation Subset: IM |
Affiliation:
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Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Animals Autoradiography Cell Division Demyelinating Diseases / chemically induced, pathology* Lysophosphatidylcholines / administration & dosage Microinjections Nerve Fibers, Myelinated / pathology Rats Rats, Inbred Strains Schwann Cells / pathology* Thymidine / pharmacology Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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P01-22849//PHS HHS; R01-14784//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Lysophosphatidylcholines; 10028-17-8/Tritium; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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