|Schwann cells can be reprogrammed to multipotency by culture.|
|PMID: 21466279 Owner: NLM Status: MEDLINE|
|Adult neural crest related-stem cells persist in adulthood, making them an ideal and easily accessible source of multipotent cells for potential clinical use. Recently, we reported the presence of neural crest-related stem cells within adult palatal ridges, thus raising the question of their localization in their endogenous niche. Using immunocytochemistry, reverse transcription-polymerase chain reaction, and correlative fluorescence and transmission electron microscopy, we identified myelinating Schwann cells within palatal ridges as a putative neural crest stem cell source. Palatal Schwann cells expressed nestin, p75(NTR), and S100. Correlative fluorescence and transmission electron microscopy revealed the exclusive nestin expression within myelinating Schwann cells. Palatal neural crest stem cells and nestin-positive Schwann cells isolated from adult sciatic nerves were able to grow under serum-free conditions as neurospheres in presence of FGF-2 and EGF. Spheres of palatal and sciatic origin showed overlapping expression pattern of neural crest stem cell and Schwann cell markers. Expression of the pluripotency factors Sox2, Klf4, c-Myc, Oct4, the NF-κB subunits p65, p50, and the NF-κB-inhibitor IκB-β were up-regulated in conventionally cultivated sciatic nerve Schwann cells and in neurosphere cultures. Finally, neurospheres of palatal and sciatic origin were able to differentiate into ectodermal, mesodermal, and endodermal cell types emphasizing their multipotency. Taken together, we show that nestin-positive myelinating Schwann cells can be reprogrammed into multipotent adult neural crest stem cells under appropriate culture conditions.|
|Darius Widera; Peter Heimann; Christin Zander; Yvonne Imielski; Meike Heidbreder; Mike Heilemann; Christian Kaltschmidt; Barbara Kaltschmidt|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-01|
|Title: Stem cells and development Volume: 20 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2011 Dec|
|Created Date: 2011-11-29 Completed Date: 2012-03-15 Revised Date: 2013-06-30|
Medline Journal Info:
|Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States|
|Languages: eng Pagination: 2053-64 Citation Subset: IM|
|Department of Cell Biology, University of Bielefeld, Bielefeld, Germany.|
|APA/MLA Format Download EndNote Download BibTex|
Cell Culture Techniques / methods*
Intermediate Filament Proteins / metabolism, ultrastructure
Ki-67 Antigen / metabolism
Mucous Membrane / cytology
Multipotent Stem Cells / cytology*, metabolism
Myelin Proteins / metabolism
Myelin Sheath / metabolism, ultrastructure
NF-kappa B / metabolism
Nerve Fibers / metabolism
Nerve Tissue Proteins / metabolism, ultrastructure
Neural Crest / cytology
Neural Stem Cells / cytology, metabolism
Palate / cytology
Pluripotent Stem Cells / cytology, metabolism
Receptors, Nerve Growth Factor / metabolism
S100 Proteins / metabolism
Schwann Cells / cytology*, metabolism, ultrastructure
Sciatic Nerve / cytology, metabolism
Synapses / metabolism
|0/Intermediate Filament Proteins; 0/Ki-67 Antigen; 0/Myelin Proteins; 0/NF-kappa B; 0/Nerve Tissue Proteins; 0/Pmp22 protein, rat; 0/Receptors, Nerve Growth Factor; 0/S100 Proteins; 0/TNFRSF16 protein, rat; 0/nestin|
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