Document Detail

Schwann cells can be reprogrammed to multipotency by culture.
MedLine Citation:
PMID:  21466279     Owner:  NLM     Status:  MEDLINE    
Adult neural crest related-stem cells persist in adulthood, making them an ideal and easily accessible source of multipotent cells for potential clinical use. Recently, we reported the presence of neural crest-related stem cells within adult palatal ridges, thus raising the question of their localization in their endogenous niche. Using immunocytochemistry, reverse transcription-polymerase chain reaction, and correlative fluorescence and transmission electron microscopy, we identified myelinating Schwann cells within palatal ridges as a putative neural crest stem cell source. Palatal Schwann cells expressed nestin, p75(NTR), and S100. Correlative fluorescence and transmission electron microscopy revealed the exclusive nestin expression within myelinating Schwann cells. Palatal neural crest stem cells and nestin-positive Schwann cells isolated from adult sciatic nerves were able to grow under serum-free conditions as neurospheres in presence of FGF-2 and EGF. Spheres of palatal and sciatic origin showed overlapping expression pattern of neural crest stem cell and Schwann cell markers. Expression of the pluripotency factors Sox2, Klf4, c-Myc, Oct4, the NF-κB subunits p65, p50, and the NF-κB-inhibitor IκB-β were up-regulated in conventionally cultivated sciatic nerve Schwann cells and in neurosphere cultures. Finally, neurospheres of palatal and sciatic origin were able to differentiate into ectodermal, mesodermal, and endodermal cell types emphasizing their multipotency. Taken together, we show that nestin-positive myelinating Schwann cells can be reprogrammed into multipotent adult neural crest stem cells under appropriate culture conditions.
Darius Widera; Peter Heimann; Christin Zander; Yvonne Imielski; Meike Heidbreder; Mike Heilemann; Christian Kaltschmidt; Barbara Kaltschmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-01
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-03-15     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2053-64     Citation Subset:  IM    
Department of Cell Biology, University of Bielefeld, Bielefeld, Germany.
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MeSH Terms
Cell Aggregation
Cell Culture Techniques / methods*
Cell Differentiation
Cell Separation
Cells, Cultured
Clone Cells
Intermediate Filament Proteins / metabolism,  ultrastructure
Ki-67 Antigen / metabolism
Mucous Membrane / cytology
Multipotent Stem Cells / cytology*,  metabolism
Myelin Proteins / metabolism
Myelin Sheath / metabolism,  ultrastructure
NF-kappa B / metabolism
Nerve Fibers / metabolism
Nerve Tissue Proteins / metabolism,  ultrastructure
Neural Crest / cytology
Neural Stem Cells / cytology,  metabolism
Nuclear Reprogramming*
Palate / cytology
Pluripotent Stem Cells / cytology,  metabolism
Receptors, Nerve Growth Factor / metabolism
S100 Proteins / metabolism
Schwann Cells / cytology*,  metabolism,  ultrastructure
Sciatic Nerve / cytology,  metabolism
Synapses / metabolism
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Ki-67 Antigen; 0/Myelin Proteins; 0/NF-kappa B; 0/Nerve Tissue Proteins; 0/Pmp22 protein, rat; 0/Receptors, Nerve Growth Factor; 0/S100 Proteins; 0/TNFRSF16 protein, rat; 0/nestin

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