Document Detail


Schizandra arisanensis extract attenuates cytokine-mediated cytotoxicity in insulin-secreting cells.
MedLine Citation:
PMID:  23239919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To explore the bioactivity of an ethanolic extract of Schizandra arisanensis (SA-Et) and isolated constituents against interleukin-1β and interferon-γ-mediated β cell death and abolition of insulin secretion.
METHODS: By employing BRIN-BD11 cells, the effects of SA-Et administration on cytokine-mediated cell death and abolition of insulin secretion were evaluated by a viability assay, cell cycle analysis, and insulin assay. The associated gene and protein expressions were also measured. In addition, the bioactivities of several peak compounds collected from the SA-Et were tested against cytokine-mediated β cell death.
RESULTS: Our results revealed that SA-Et dose-dependently ameliorated cytokine-mediated β cell death and apoptosis. Instead of suppressing inducible nitric oxide synthase/nitric oxide cascade or p38MAPK activity, suppression of stress-activated protein kinase/c-Jun NH2-terminal kinase activity appeared to be the target for SA-Et against the cytokine mix. In addition, SA-Et provided some insulinotropic effects which re-activated the abolished insulin exocytosis in cytokine-treated BRIN-BD11 cells. Finally, schiarisanrin A and B isolated from the SA-Et showed a dose-dependent protective effect against cytokine-mediated β cell death.
CONCLUSION: This is the first report on SA-Et ameliorating cytokine-mediated β cell death and dysfunction via anti-apoptotic and insulinotropic actions.
Authors:
Yi-Shin Hsu; Yao-Haur Kuo; Hui-Ling Cheng; Peter R Flatt; Hui-Kang Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  18     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-14     Completed Date:  2013-12-31     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  6809-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Cell Cycle / drug effects
Cell Death*
Cell Line
Cell Survival
Chromatography, High Pressure Liquid
Cytokines / metabolism*
Glucose / metabolism
Insulin / metabolism,  secretion
Insulin-Secreting Cells / drug effects*,  metabolism
Interferon-gamma / metabolism
Interleukin-1beta / metabolism
Plant Extracts / pharmacology*
Rats
Schisandra / chemistry*
p38 Mitogen-Activated Protein Kinases / metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/Insulin; 0/Interleukin-1beta; 0/Plant Extracts; 82115-62-6/Interferon-gamma; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; IY9XDZ35W2/Glucose
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