| Schistosoma mansoni: eggshell formation is regulated by pH and calcium. | |
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MedLine Citation:
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PMID: 1915744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protein precursors of the schistosome eggshell are synthesized and packaged into secretory vesicles in the vitelline cells. These vesicles appear to contain an emulsion of eggshell precursor material. Evidence is presented to show that these secretory vesicles are acidic as in other systems and that this acidity stabilizes the emulsion and prevents the eggshell cross-linking reactions from occurring. Alkalinizing treatments trigger eggshell formation within the secretory vesicles as shown by (1) the induction of autofluorescence and (2) by electron microscopy which shows that the eggshell precursors have aggregated within the secretory vesicles into spherical particles bearing microspines. These aggregates formed in the secretory vesicles were isolated and shown to have the same protease resistance and amino acid composition as authentic eggshell. The calcium ionophore A23187 induces scattered autofluorescence in intact female worms which electron micrographs show to be due to exocytosis of eggshell material. Based on these observations we propose a model for the formation of schistosome eggshell and suggest that it may apply to all trematodes in which the eggshell precursors are present as stable emulsions in the secretory vesicles of the vitelline cells. |
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Authors:
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K E Wells; J S Cordingley |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Experimental parasitology Volume: 73 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-11-07 Completed Date: 1991-11-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 295-310 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Wyoming, Laramie 82071. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonium Chloride
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pharmacology Animals Calcimycin / pharmacology Calcium / physiology* Cytoplasmic Granules / ultrastructure Egg Proteins / metabolism* Female Helminth Proteins / metabolism* Hydrogen-Ion Concentration Microscopy, Electron Microscopy, Fluorescence Monensin / pharmacology Monophenol Monooxygenase / metabolism Ovum / physiology*, ultrastructure Protein Precursors / metabolism* Schistosoma mansoni / physiology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Egg Proteins; 0/Helminth Proteins; 0/Protein Precursors; 12125-02-9/Ammonium Chloride; 17090-79-8/Monensin; 52665-69-7/Calcimycin; 7440-70-2/Calcium; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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