Document Detail

A Schistosoma mansoni Pad1 homologue stabilizes c-Jun.
MedLine Citation:
PMID:  11985875     Owner:  NLM     Status:  MEDLINE    
We report the cloning and functional analysis of a Pad1 homologue (SmPOH) from Schistosoma mansoni. SmPOH encodes a protein of approximately 35 kDa with high amino acid identities to yeast Pad1 (65%) and its human homologue, POH1 (78%). Members of the Pad1 family are subunits of the 26S proteasome and have been implicated as positive modulators of transcription in yeast. Recombinant SmPOH expressed in COS7 cells exhibited a punctate pattern of distribution throughout the cytoplasm and nucleus, predominantly in the nuclear periphery, a distribution consistent with that of the cellular proteasome. Transient overexpression of SmPOH in COS7 cells caused a dose-dependent stimulation in AP-1 transcriptional activity, as determined by a reporter gene assay. This effect was associated with a pronounced increase in the levels of cellular c-Jun. In vitro degradation assays further demonstrated that SmPOH specifically decreased the rate of c-Jun degradation in a dose dependent manner. Taken together, these results suggest that SmPOH, and possibly other related Pad1 proteins, function as positive modulators of transcription by increasing the stability of cellular c-Jun, making elevated amounts of this protein available for transactivation of AP-1-responsive genes.
Joseph F Nabhan; Fadi F Hamdan; Paula Ribeiro
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Publication Detail:
Type:  Corrected and Republished Article; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  121     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-02     Completed Date:  2002-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  163-72     Citation Subset:  IM    
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Québec, Canada.
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MeSH Terms
Amino Acid Sequence
COS Cells
Caenorhabditis elegans Proteins
Cloning, Molecular
Drug Resistance, Multiple*
Gene Expression Regulation
Helminth Proteins / chemistry,  genetics*,  metabolism*
Membrane Proteins / genetics*,  metabolism
Molecular Sequence Data
Proteasome Endopeptidase Complex
Proto-Oncogene Proteins c-jun / metabolism*
Schistosoma mansoni / genetics,  metabolism*
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trans-Activators / genetics,  metabolism
Transcription Factor AP-1 / genetics,  metabolism
Transcription, Genetic
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Helminth Proteins; 0/Membrane Proteins; 0/PSMD14 protein, human; 0/Pad-1 protein, C elegans; 0/Proto-Oncogene Proteins c-jun; 0/SmPOH protein, Schistosoma mansoni; 0/Trans-Activators; 0/Transcription Factor AP-1; EC Endopeptidase Complex
Corrected and Republished From:
Mol Biochem Parasitol. 2001 Sep 3;116(2):209-18   [PMID:  11522353 ]

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