Document Detail

Schistosoma japonicum: ultrastructural localization of a hemoglobinase using mercury labeled pepstatin.
MedLine Citation:
PMID:  3527739     Owner:  NLM     Status:  MEDLINE    
Mercury labeled pepstatin was used to demonstrate the site of a pepstatin sensitive hemoglobinase in paraformaldehyde fixed adult Schistosoma japonicum. Pepstatin was covalently attached to glutathione using dicyclohexylcarbodiimide followed by addition to methyl mercury chloride. Deposition of mercury was observed in lipid-like globules and autophagic vacuoles in the gastrodermis. Control studies were negative in all instances. These results complement previous cytochemical studies on the distribution of other acid hydrolases in the gastrodermis of schistosomes. It is hypothesized that this pepstatin sensitive enzyme probably belongs to the carboxyl class of proteinases.
B J Bogitsh; K F Kirschner
Related Documents :
22177309 - Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid h...
6153929 - Ultrastructural observations on the cell surface of the intestinal epithelium of the ne...
12946139 - Efficient synthesis of enantiopure pyrrolizidinone amino acid.
17640699 - Differences in the molecular composition of fulvic acid size fractions detected by size...
12137279 - Organic compounds in carbonaceous meteorites.
23087649 - Outlook: membrane junctions enable the metabolic trapping of fatty acids by intracellul...
16643869 - Pectin-like acidic polysaccharide from panax ginseng with selective antiadhesive activi...
16917129 - Phosphatidic acid osmotically destabilizes lysosomes through increased permeability to ...
17516469 - Porphomethene inhibitor of uroporphyrinogen decarboxylase: analysis by high-performance...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental parasitology     Volume:  62     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-10-15     Completed Date:  1986-10-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  211-5     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aspartic Acid Endopeptidases
Endopeptidases / analysis*
Hemoglobins / metabolism*
Methylmercury Compounds
Pepstatins / pharmacology
Schistosoma japonicum / enzymology*,  ultrastructure
Reg. No./Substance:
0/Hemoglobins; 0/Methylmercury Compounds; 0/Pepstatins; 11076-29-2/Streptomyces pepsin inhibitor; 115-09-3/methylmercuric chloride; 39324-30-6/pepstatin; EC 3.4.-/Endopeptidases; EC 3.4.23.-/Aspartic Acid Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Plasmodium berghei: histology, immunocytochemistry, and ultrastructure of the placenta in rodent mal...
Next Document:  Schistosoma mansoni: surface membrane stability in vitro and in vivo.