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Scavenging of hydroxyl radical by catecholamines.
MedLine Citation:
PMID:  22238226     Owner:  NLM     Status:  Publisher    
The direct effects of the four catecholamines (CATs), adrenaline (A), noradrenaline (NA), dopamine (D) and isoproterenol (I), on free radicals were investigated using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH(•) ) and hydroxyl radial (HO(•) ). The CATs examined were found to inhibit the ESR signal intensity of DPPH(•) in a dose-dependent manner over the range 0.1-2.5 mmol/L in the following order: NA > A > I > D, with IC(50)  = 0.30 ± 0.03 for noradrenaline and IC(50)  = 0.86 ± 0.02 for dopamine. Hydroxyl radicals were produced using a Fenton reaction in the presence of the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and ESR technique was applied to detect the CATs reactivity toward the radicals. The reaction rates constant (k(r) ) of CATs with HO(•) were found to be in the order of 10(9)  L/mol/s, and the k(r) value for noradrenaline was the highest (k(r)  = 8.4 × 10(9)  L/mol/s). The CATs examined exhibited also a strong decrease in the light emission (62-73% at 1 mmol/L concentration and 79-89% at 2 mmol/L concentration) from a Fenton-like reaction. These reactions may be relevant to the biological action of these important polyphenolic compounds. Copyright © 2012 John Wiley & Sons, Ltd.
Aleksandra Kładna; Paweł Berczyński; Irena Kruk; Teresa Michalska; Hassan Y Aboul-Enein
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-12
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  -     ISSN:  1522-7243     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Department of Medical History and Ethics, Pomeranian Medical University of Szczecin, Poland.
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