Document Detail


Scanning near-field optical microscopy in cell biology and microbiology.
MedLine Citation:
PMID:  9764739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Scanning a point light source in close proximity over a sample and recording the scattered or transmitted light intensity point by point allows one to record optical images with a resolution not limited by diffraction. An overview of this technique called scanning near-field optical microscopy (SNOM or NSOM) is given with emphasis on cell- and microbiology. After an introduction, where the basic features of the technique are explained, illustrative examples are presented, such as a HeLa cell, fluorescence labelled human chromosomes, super resolution fluorescence imaging, single molecule imaging and fluorescence resonance energy transfer between a single pair of dye molecules.
Authors:
A J Meixner; H Kneppe
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Publication Detail:
Type:  Comparative Study; Journal Article; Review    
Journal Detail:
Title:  Cellular and molecular biology (Noisy-le-Grand, France)     Volume:  44     ISSN:  0145-5680     ISO Abbreviation:  Cell. Mol. Biol. (Noisy-le-grand)     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-12-17     Completed Date:  1998-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9216789     Medline TA:  Cell Mol Biol (Noisy-le-grand)     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  673-88     Citation Subset:  IM    
Affiliation:
Universität-Gesamthochschule Siegen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human
Fluorescent Dyes
Hela Cells / ultrastructure
Humans
In Situ Hybridization, Fluorescence
Microbiological Techniques / instrumentation
Microscopy / instrumentation*,  methods*
Microscopy, Atomic Force / instrumentation,  methods
Microscopy, Electron, Scanning / instrumentation,  methods
Molecular Biology / methods
Spectrometry, Fluorescence / methods
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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