Document Detail


Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions.
MedLine Citation:
PMID:  23421780     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.
Authors:
Chiao-Chen Chen; Yi Zhou; Celeste A Morris; Jianghui Hou; Lane A Baker
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-18
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-02     Completed Date:  2013-11-18     Revised Date:  2014-04-04    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3621-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Claudin-2 / genetics,  metabolism*
Dogs
Electrochemical Techniques / instrumentation*
Epithelium / metabolism*
Equipment Design
Ion Transport
Ions / metabolism*
Microscopy / instrumentation*
Permeability
Tight Junctions / metabolism*
Grant Support
ID/Acronym/Agency:
1R21DK082990/DK/NIDDK NIH HHS; P30DK079333/DK/NIDDK NIH HHS; R01DK084059/DK/NIDDK NIH HHS; R21 DK082990/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Claudin-2; 0/Ions
Comments/Corrections

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