Document Detail


Scanning ion analytical microscopy for high-resolution detection of 5-bromo-2'-deoxyuridine incorporation in metaphase chromosomes.
MedLine Citation:
PMID:  9024974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the possibilities of using scanning ion analytical microscopy (SIAM) to detect bromine in human metaphase chromosomes. The experiments were performed after incorporation of the thymidine analogue, 5-bromo-2'-deoxyuridine (BrdU), into the DNA or by in situ hybridization of a BrdU-labelled probe for the subcentromeric repeated DNA sequences. The possibilities offered by this microanalytical method were compared with immunofluorescent staining techniques. Well-defined maps of bands containing bromide were obtained with metaphase chromosomes that had incorporated BrdU during the late S-phase. Their patterns were similar to the labelling obtained by immunofluorescence. In addition, SIAM reveals the presence of bromine within constitutive heterochromatic regions in which BrdU is poorly detected by immunofluorescence. The comparison of the 12C14N, 31P and 81Br maps of controls and fluorescence plus Giemsa (FPG) metaphase chromosomes shows the loss of bromide from DNA during this treatment. SIAM emerges as a new powerful microanalytical technology for investigating chromosome structure further.
Authors:
C A Bourgeois; R Dennebouy; A Gibaud; M Gerbault-Seureau; B Malfoy; G Slodzian; P Galle; B Dutrillaux
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  4     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-04-03     Completed Date:  1997-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  574-82     Citation Subset:  IM    
Affiliation:
Institut Curie-CNRS UMR 147, Paris, France. claire.bourgeois@curie.fr
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MeSH Terms
Descriptor/Qualifier:
Bromine / analysis
Bromodeoxyuridine / analysis*
Cells, Cultured
Chromosomes / chemistry*
DNA / chemistry
Fluorescent Antibody Technique
Humans
In Situ Hybridization, Fluorescence
Lymphocytes
Mass Spectrometry / methods
Metaphase
Microscopy / methods*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine; 7726-95-6/Bromine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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