Document Detail


Scanning electron microscopy with an ionic liquid reveals the loss of mitotic protrusions of cells during the epithelial-mesenchymal transition.
MedLine Citation:
PMID:  21413103     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Epithelial-mesenchymal transition (EMT) is a key event in cancer metastasis and is characterized by increase in cell motility, increase in expression of mesenchymal cell markers, loss of proteins from cell-to-cell junction complexes, and changes in cell morphology. Here, the morphological effects of a representative EMT inducer, transforming growth factor (TGF)-β1, were investigated in human lung adenocarcinoma (A549) cells and pancreatic carcinoma (Panc-1) cells. TGF-β1 caused morphological changes characteristic of EMT, and immunostaining showed loss of E-cadherin from cell-to-cell junction complexes in addition to the upregulation of the mesenchymal marker vimentin. During scanning electron microscopy (SEM) with an ionic liquid, we observed EMT-specific morphological changes, including the formation of various cell protrusions. Interestingly, filopodia in mitotic cells were clearly observed by SEM, and the number of these filopodia in TFG-β1-treated mitotic cells was reduced significantly. We conclude that this reduction in such mitotic protrusions is a novel effect of TGF-β1 and may contribute to EMT. Microsc. Res. Tech., 2011. © 2011 Wiley-Liss, Inc.
Authors:
Yasuhito Ishigaki; Yuka Nakamura; Teruaki Takehara; Takeo Shimasaki; Takanori Tatsuno; Fumihide Takano; Yoshimichi Ueda; Yoshiharu Motoo; Tsutomu Takegami; Hideaki Nakagawa; Susumu Kuwabata; Noriko Nemoto; Naohisa Tomosugi; Shichiro Miyazawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-16
Journal Detail:
Title:  Microscopy research and technique     Volume:  -     ISSN:  1097-0029     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Medical Research Institute, Kanazawa Medical University, Uchinada-Machi, Kahoku-Gun, Japan.
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