| Scanning electron microscopy with an ionic liquid reveals the loss of mitotic protrusions of cells during the epithelial-mesenchymal transition. | |
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MedLine Citation:
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PMID: 21413103 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Epithelial-mesenchymal transition (EMT) is a key event in cancer metastasis and is characterized by increase in cell motility, increase in expression of mesenchymal cell markers, loss of proteins from cell-to-cell junction complexes, and changes in cell morphology. Here, the morphological effects of a representative EMT inducer, transforming growth factor (TGF)-β1, were investigated in human lung adenocarcinoma (A549) cells and pancreatic carcinoma (Panc-1) cells. TGF-β1 caused morphological changes characteristic of EMT, and immunostaining showed loss of E-cadherin from cell-to-cell junction complexes in addition to the upregulation of the mesenchymal marker vimentin. During scanning electron microscopy (SEM) with an ionic liquid, we observed EMT-specific morphological changes, including the formation of various cell protrusions. Interestingly, filopodia in mitotic cells were clearly observed by SEM, and the number of these filopodia in TFG-β1-treated mitotic cells was reduced significantly. We conclude that this reduction in such mitotic protrusions is a novel effect of TGF-β1 and may contribute to EMT. Microsc. Res. Tech., 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Yasuhito Ishigaki; Yuka Nakamura; Teruaki Takehara; Takeo Shimasaki; Takanori Tatsuno; Fumihide Takano; Yoshimichi Ueda; Yoshiharu Motoo; Tsutomu Takegami; Hideaki Nakagawa; Susumu Kuwabata; Noriko Nemoto; Naohisa Tomosugi; Shichiro Miyazawa |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-16 |
Journal Detail:
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Title: Microscopy research and technique Volume: - ISSN: 1097-0029 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Medical Research Institute, Kanazawa Medical University, Uchinada-Machi, Kahoku-Gun, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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