Document Detail

Scanning electron microscopy and transmission electron microscopy aspects of synergistic antitumor activity of vitamin C - vitamin K3 combinations against human prostatic carcinoma cells.
MedLine Citation:
PMID:  8553014     Owner:  NLM     Status:  MEDLINE    
A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K3 ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10- to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following a 1 hour treatment with Vit C, Vit K3, or Vit C/K3 combined at their 50% cytotoxic dose. Our morphological data suggest that vitamin treatment with individual vitamins affects the cytoskeleton, the mitochondria, and other membranous components of the cell. Treatment with the vitamin combination appears to potentiate the effects of the individual vitamin treatment. Specifically, there are abundant necrotic cells. The surviving cells display morphological defects characteristic of cell injury.
J Gilloteaux; J M Jamison; M Venugopal; D Giammar; J L Summers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scanning microscopy     Volume:  9     ISSN:  0891-7035     ISO Abbreviation:  Scanning Microsc.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1996-02-20     Completed Date:  1996-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8704616     Medline TA:  Scanning Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-73     Citation Subset:  IM    
Department of Anatomy, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Ascorbic Acid / pharmacology*
Carcinoma / drug therapy*,  pathology
Cell Membrane / drug effects,  ultrastructure
Coloring Agents
Cytoskeleton / drug effects,  ultrastructure
Drug Synergism
Drug Therapy, Combination
Microscopy, Electron
Microscopy, Electron, Scanning
Mitochondria / drug effects,  ultrastructure
Prostatic Neoplasms / drug therapy*,  pathology
Tetrazolium Salts
Tumor Cells, Cultured
Vitamin K / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Coloring Agents; 0/Tetrazolium Salts; 0/Thiazoles; 12001-79-5/Vitamin K; 298-93-1/thiazolyl blue; 50-81-7/Ascorbic Acid

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