Document Detail


Scanning electron microscopic and cytochemical analyses of alveolar and peritoneal macrophages of mice, dogs and cats.
MedLine Citation:
PMID:  9819771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alveolar and peritoneal macrophages (MPs) of mouse, dog and cat were compared in relation to their scanning electron microscopic features and the lysosomal activities of nonspecific esterase, acid phosphatase, and beta-glucuronidase. The long spindle shape of peritoneal MPs differed from the spherical form of alveolar MPs in all species. There was no difference in the morphological findings among the three animals. Murine alveolar and peritoneal MPs were strongly positive for all three enzymes. Canine and feline alveolar and peritoneal MPs were strongly positive for acid phosphatase and beta-glucuronidase, but weakly positive for nonspecific esterase. These results strongly suggest that acid phosphatase and beta-glucuronidase can be used as markers of the MPs in healthy dogs and cats.
Authors:
Y Okamoto; T Tanigawa; Y Tanaka; T Kameie; A Iino; N Kasagi; S Minami
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  60     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-02-02     Completed Date:  1999-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1145-8     Citation Subset:  IM    
Affiliation:
Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Animals
Carboxylesterase
Carboxylic Ester Hydrolases / metabolism
Cats / physiology*
Dogs / physiology*
Female
Glucuronidase / metabolism
Histocytochemistry
Lysosomes / enzymology
Macrophages, Alveolar / ultrastructure*
Macrophages, Peritoneal / ultrastructure*
Mice / physiology*
Microscopy, Electron, Scanning / veterinary*
Species Specificity
Chemical
Reg. No./Substance:
EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.1/Carboxylesterase; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.31/Glucuronidase

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