Document Detail

Scanning electrochemical microscopy of model neurons: imaging and real-time detection of morphological changes.
MedLine Citation:
PMID:  12585485     Owner:  NLM     Status:  MEDLINE    
Living PC12 cells, a model cell type for studying neuronal function, were imaged using the negative feedback mode of a scanning electrochemical microscope (SECM). Six biocompatible redox mediators were successfully identified from a large pool of candidates and were then used for imaging PC12 cells before and after exposure to nerve growth factor (NGF). When exposed to NGF, cells differentiate into a neuron phenotype by growing narrow neurites (1-2 microm wide) that can extend > 100 microm from the cell proper. We demonstrate that carbon fiber electrodes with reduced tip diameters can be used for imaging both the cell proper and these neurites. Regions of decreased current, possibly resulting from raised features not identifiable by light microscopy, are clearly evident in the SECM images. Changes in the morphology of undifferentiated PC12 cells could be detected in real time with the SECM. After exposure to hypotonic and hypertonic solutions, reversible changes in cell height of <2 microm were measured.
Johanna M Liebetrau; Heather M Miller; John E Baur; Sara A Takacs; Vipavee Anupunpisit; Paul A Garris; David O Wipf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  75     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  563-71     Citation Subset:  IM    
Department of Chemistry, Illinois State University, Normal, Illinois 61790-4160, USA.
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MeSH Terms
Diagnostic Imaging
Electrochemistry / instrumentation
Microscopy, Electron, Scanning / instrumentation*
Nerve Growth Factor / pharmacology
Neurons / cytology*,  drug effects
PC12 Cells
Reg. No./Substance:
9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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