Document Detail

Scale-up of a new bacterial mannitol production process.
MedLine Citation:
PMID:  12790644     Owner:  NLM     Status:  MEDLINE    
D-Mannitol is a sugar alcohol with applications in chemistry, food and pharmaceutical industries, and medicine. Commercially, mannitol is produced by catalytic hydrogenation. Although this process is widely used, it is not optimal for mannitol production. New processes, including chemical, enzymatic, and microbial processes, are frequently developed and evaluated against the existing hydrogenation processes. In earlier papers, we have described the identification of a food-grade lactic acid bacterium strain, Leuconostoc mesenteroides ATCC-9135, with efficient mannitol production capabilities and the development and optimization of a new bioprocess in which the strain was applied. The new bioprocess is simple. It requires a reduced bioreactor with the following features: sterilization, pH and T control (at mild conditions), and slow mixing. The contamination risk of the new bioprocess is low, and the downstream processing protocol comprises simple, widely used unit operations: evaporation, crystallization, crystal separation, and drying. On a 2-L laboratory scale, high mannitol yields from fructose (93-97%) and volumetric mannitol productivities (>20 g L(-1) h(-1)) were achieved. In this paper, the scalability of the new bioprocess was tested on a small pilot scale (100 L). In the pilot plant, production levels were achieved similar to those in the laboratory. Also, high-purity mannitol crystals were obtained at similar yield levels. The results presented in this paper indicate that the new bioprocess can easily be scaled-up to an industrial scale and that the production levels achieved with it are comparable to the catalytic hydrogenation processes.
F Niklas W von Weymarn; Kristiina J Kiviharju; Seppo T Jääskeläinen; Matti S A Leisola
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Biotechnology progress     Volume:  19     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-06-06     Completed Date:  2004-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  815-21     Citation Subset:  IM    
Laboratory of Bioprocess Engineering, Department of Chemical Technology, P. O. Box 6100, Helsinki University of Technology, FIN-02015 Helsinki, Finland.
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MeSH Terms
Bioreactors / microbiology*
Cell Culture Techniques / instrumentation,  methods*
Leuconostoc / classification,  growth & development*,  metabolism*
Mannitol / isolation & purification*,  metabolism*
Membranes, Artificial
Pilot Projects
Species Specificity
Reg. No./Substance:
0/Membranes, Artificial; 69-65-8/Mannitol

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