Document Detail

Scale-up of microbubble dispersion generator for aerobic fermentation.
MedLine Citation:
PMID:  12109817     Owner:  NLM     Status:  MEDLINE    
A laboratory-scale microbubble dispersion (MBD) generator was shown to improve oxygen transfer to aerobic microorganisms when coupled to the conventional air-sparger. However, the process was not demonstrated on a large scale to prove its practical application. We investigated the scale-up of a spinning-disk MBD generator for the aerobic fermentation of Saccharomyces cerevisiae (baker's yeast). A 1-L spinning-disk MBD generator was used to supply air for 1- and 50-L working volume fermentation of baker's yeast. For the two levels investigated, the MBD generator maintained an adequate supply of surfactant-stabilized air microbubbles to the microorganisms at a relatively low agitation rate (150 rpm). There was a significant improvement in oxygen transfer to the microorganism relative to the conventional sparger. The volumetric mass transfer coefficient, kLa, for the MBD system at 150 rpm was 765 h(-1) compared to 937 h(-1) for the conventional sparger at 500 rpm. It is plausible to surmise that fermentation using larger working volumes may further improve the kLa values and the dissolved oxygen (DO) levels because of longer hold-up times and, consequently, improve cell growth. There was no statistically significant difference between the cell mass yield on substrate (0.43 g/g) under the MBD regime at an agitation rate of 150 rpm and that achieved for the conventional air-sparged system (0.53 g/g) at an agitation rate of 500 rpm. The total power consumption per unit volume of broth in the 50-L conventional air-sparged system was threefold that for the MBD unit for a similar product yield. Practical application of the MBD technology can be expected to reduce power consumption and therefore operating costs for aerobic fermentation.
P Hensirisak; P Parasukulsatid; F A Agblevor; J S Cundiff; W H Velander
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  101     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-11     Completed Date:  2002-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  211-27     Citation Subset:  IM    
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
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MeSH Terms
Equipment Design
Saccharomyces cerevisiae / metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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