| Saxifragifolin B from Androsace umbellata induced apoptosis on human hepatoma cells. | |
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MedLine Citation:
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PMID: 17765201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bioassay-guided phytochemical study of Androsace umbellata led to the successful isolation of saxifragifolin B (SB) for the first time. The anti-tumor effect of SB was firstly reported that it was shown to have potent cytotoxicity on human hepatoma HepG2 cells with IC50 value of 11.9 microM at 24 h. Mechanistic studies were conducted, the accumulation of sub-G1 population and the externalization of phosphatidylserine suggested that SB exerted its cytotoxic effect by induction of programmed cell death, which was confirmed by activation of PARP and caspase-3. Furthermore, SB-induced apoptosis on HepG2 cells was mediated by activation of caspase-8 and -9, mitochondrial membrane potential (Deltapsim) collapse and the leakage of cytochrome c. In summary, this study provided evidence that SB isolated from A. umbellata could induce apoptosis on human hepatoma HepG2 cells and described the molecular mechanism. Our finding revealed the potential of SB as new chemotherapeutic agent for human hepatoma. |
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Authors:
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Dong-Mei Zhang; Ying Wang; Ming-Kuen Tang; Yuet-Wa Chan; Hung-Ming Lam; Wen-Cai Ye; Kwok-Pui Fung |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-23 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 362 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-11 Completed Date: 2007-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 759-65 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Medicine, Room 603, Mong Man Wai Building, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Carcinoma, Hepatocellular / metabolism, pathology* Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Line, Tumor Cell Proliferation Dose-Response Relationship, Drug Humans Inhibitory Concentration 50 Membrane Potential, Mitochondrial Membrane Potentials Molecular Conformation Plant Extracts / chemistry, pharmacology* Plants / metabolism* Saponins / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 0/Saponins; 0/saxifragifolin B; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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