Document Detail


Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture.
MedLine Citation:
PMID:  15945276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the effects of saturated free fatty acid, palmitic acid (PA), on hepatocytes, we administered PA to rat hepatocytes. We demonstrated that PA inhibited the cell growth as a dose- and time-dependent manner in rat hepatocytes. PA-induced morphological changes including swelling, membrane dissolution and formation of debris, and apoptosis with appearance of sub-G1 fraction determined by cell cycle analysis after treatment for 4 days. The level of Bcl-2 was slightly decreased, in contrast, the level of Bax elevated markedly, which resulted in a significant decrease of Bcl-2/Bax ratio after PA treatment on HepG2 cells. These findings demonstrated that PA induces cell death on hepatocytes, perhaps via mitochondria-mediated apoptosis. Furthermore, the present study indicates that PA's cell toxicity may play important roles in the transition from steatosis to steatohepatitis in human especially with obesity.
Authors:
Jun Ji; Li Zhang; Ping Wang; Yi-Ming Mu; Xiao-Yu Zhu; Yuan-Yuan Wu; Huan Yu; Bin Zhang; Shu-Min Chen; Xi-Zuo Sun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie     Volume:  56     ISSN:  0940-2993     ISO Abbreviation:  Exp. Toxicol. Pathol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-06-10     Completed Date:  2005-08-16     Revised Date:  2006-10-30    
Medline Journal Info:
Nlm Unique ID:  9208920     Medline TA:  Exp Toxicol Pathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  369-76     Citation Subset:  IM    
Affiliation:
Department of Central Laboratory, Dalian Municipal Central Hospital, Xuegong Street 42, Shahekou-qu, Dalian 116033, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A5 / metabolism
Apoptosis / drug effects*
Cell Enlargement / drug effects
Cell Separation
Cell Survival / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Fetus / cytology*
Flow Cytometry
Hepatocytes / drug effects*,  metabolism,  pathology
Palmitic Acid / toxicity*
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Rats
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Annexin A5; 0/Bax protein, rat; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 57-10-3/Palmitic Acid

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