Document Detail

Saturable attachment sites for type 3 mammalian reovirus on murine L cells and human HeLa cells.
MedLine Citation:
PMID:  6398564     Owner:  NLM     Status:  MEDLINE    
Attachment of [35S]methionine-labelled mammalian type 3 reovirus to murine L cells and human HeLa cells was studied under equilibrium conditions. Cellular attachment sites could be completely saturated with 35S-labelled reovirus, indicating that specific attachment sites for reovirus are present on the surface of these cells. We calculated that L cells possess about 86000-105000 attachment sites per cell while HeLa cells possess about 126000-147000 sites per cell for type 3 reovirus. Unlabelled reovirus was highly efficient in competing for attachment by 35S-labelled reovirus to the saturable attachment sites of both L and HeLa cells, further indicating the specificity of the interaction. We also found that unlabelled reovirus competed equally well for both binding and internalization of 35S-labelled reovirus into murine L cells, suggesting that the L cell attachment site may serve as a virus entry site. Phospholipase digestion of L cells had no effect on subsequent reovirus attachment, while treatment of L cells with moderate concentrations of bromelain (but not trypsin, proteinase K or pronase) and Vibrio cholerae neuraminidase reproducibly decreased subsequent reovirus attachment. These results and those of others (Epstein et al., 1984, Virology 133, 46-55) suggest that mammalian reoviruses attach to specific cell surface receptors on at least two species of mammalian cells to initiate the infectious cycle.
J R Gentsch; J W Hatfield
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virus research     Volume:  1     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1984  
Date Detail:
Created Date:  1985-06-06     Completed Date:  1985-06-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  401-14     Citation Subset:  IM    
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MeSH Terms
Binding, Competitive
Hela Cells
L Cells (Cell Line) / drug effects
Mammalian orthoreovirus 3 / physiology*
Neuraminidase / pharmacology
Peptide Hydrolases / pharmacology
Receptors, Virus / analysis*
Reoviridae / physiology*
Sulfur Radioisotopes
Type C Phospholipases / pharmacology
Grant Support
Reg. No./Substance:
0/Receptors, Virus; 0/Sulfur Radioisotopes; EC 3.1.4.-/Type C Phospholipases; EC; EC 3.4.-/Peptide Hydrolases

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