Document Detail


Satellite NG2 progenitor cells share common glutamatergic inputs with associated interneurons in the mouse dentate gyrus.
MedLine Citation:
PMID:  18650338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several studies have provided evidence that NG2-expressing (NG2(+)) progenitor cells are anatomically associated to neurons in gray matter areas. By analyzing the spatial distribution of NG2(+) cells in the hilus of the mouse dentate gyrus, we demonstrate that NG2(+) cells are indeed closely associated to interneurons. To define whether this anatomical proximity reflected a specific physiological interaction, we performed patch-clamp recordings on hilar NG2(+) cells and interneurons between 3 and 21 postnatal days. We first observed that hilar NG2(+) cells exhibit spontaneous glutamatergic EPSCs (sEPSCs) whose frequency and amplitude increase during the first 3 postnatal weeks. At the same time, the rise time and decay time of sEPSCs significantly decreased, suggesting that glutamatergic synapses in NG2(+) cells undergo a maturation process that is reminiscent of what has been reported in neurons during the same time period. We also observed that hilar interneurons and associated NG2(+) cells are similarly integrated into the local network, receiving excitatory inputs from both granule cells and CA3 pyramidal neurons. By performing pair recordings, we found that bursts of activity induced by GABAergic antagonists were strongly synchronized between both cell types and that the amplitude of these bursts was positively correlated. Finally, by applying carbachol to increase EPSC activity, we observed that closely apposed cells were more likely to exhibit synchronized EPSCs than cells separated by >200 microm. The finding that NG2(+) cells are sensing patterns of activity arising in closely associated neurons suggests that NG2(+) cell function is finely regulated by the local network.
Authors:
Jean-Marie Mangin; Albrecht Kunze; Ramesh Chittajallu; Vittorio Gallo
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  28     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-24     Completed Date:  2008-09-02     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7610-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Animals
Animals, Newborn
Antigens / metabolism*
Benzothiadiazines / pharmacology
Cyclopropanes / pharmacology
Dentate Gyrus / cytology*
Dose-Response Relationship, Radiation
Electric Stimulation
Excitatory Amino Acid Antagonists / pharmacology
Excitatory Postsynaptic Potentials / drug effects,  physiology,  radiation effects
GABA Antagonists / pharmacology
Glutamic Acid / metabolism*
Glycine / analogs & derivatives,  pharmacology
Green Fluorescent Proteins / genetics
Interneurons / physiology*
Mice
Mice, Transgenic
Patch-Clamp Techniques / methods
Phosphopyruvate Hydratase / metabolism
Phosphoric Diester Hydrolases / genetics
Picrotoxin / pharmacology
Proteoglycans / metabolism*
Satellite Cells, Perineuronal / physiology*
Sodium Channel Blockers / pharmacology
Stem Cells
Tetrodotoxin / pharmacology
Grant Support
ID/Acronym/Agency:
P30 HD040677/HD/NICHD NIH HHS; P30 HD040677-08/HD/NICHD NIH HHS; P30HD40677/HD/NICHD NIH HHS; R01 NS045702/NS/NINDS NIH HHS; R01 NS045702-05/NS/NINDS NIH HHS; R01NS045702/NS/NINDS NIH HHS; R21 NS050463/NS/NINDS NIH HHS; R21 NS050463-02/NS/NINDS NIH HHS; R21NS050463/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens; 0/Benzothiadiazines; 0/Cyclopropanes; 0/Excitatory Amino Acid Antagonists; 0/GABA Antagonists; 0/Proteoglycans; 0/Sodium Channel Blockers; 0/chondroitin sulfate proteoglycan 4; 0/enhanced green fluorescent protein; 124-87-8/Picrotoxin; 147336-22-9/Green Fluorescent Proteins; 147782-19-2/2-(2,3-dicarboxycyclopropyl)glycine; 3KX376GY7L/Glutamic Acid; 4368-28-9/Tetrodotoxin; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.37/2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; EC 3.1.4.37/Cnp protein, mouse; EC 4.2.1.11/Phosphopyruvate Hydratase; P71U09G5BW/cyclothiazide; TE7660XO1C/Glycine
Comments/Corrections

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