Document Detail

Sarcomatoid carcinoma represents a complete phenotype with various pathways of epithelial mesenchymal transition.
MedLine Citation:
PMID:  23533257     Owner:  NLM     Status:  Publisher    
AIMS: Sarcomatoid carcinoma (SC) is considered to be a result of the sarcomatoid change of epithelial carcinoma. However, epithelial-mesenchymal transition (EMT) in SC has been insufficiently studied. METHODS: We evaluated the expression patterns of EMT-related phenotypic markers with transcription factors in 27 SCs originating from various organs, and we investigated the phenotypic characteristics of SCs classified as complete, incomplete or wild-type. We further analysed correlations between EMT-related phenotype markers and transcription factors. RESULTS: Epithelial markers (E-cadherin, claudin-3 and claudin-4) were consistently down-regulated, whereas mesenchymal markers (S100A4, α-smooth muscle actin (SMA), vimentin, PDGFRα and β-catenin) were variously expressed except for vimentin. EMT-related transcription factors (SIP1, Snail1, Slug, Twist1, ZEP1 and Oct-4) also showed various expression patterns. The expression patterns of phenotypic markers showed that most SCs (22/27, 81.5%, 95% CI 65.8 to 97.1%) had complete EMT phenotypes, whereas the remaining 5 (18.5%, 95% CI 2.8 to 24.1%) were of incomplete type. Unsupervised hierarchical clustering analysis revealed that SCs were clustered into several subgroups by EMT-related protein expression pattern. Twist1 positivity was significantly concordant with α-SMA positivity (κ value: 0.908; 95% CI 0.73 to 1.00, p<0.001, adjusted p<0.001). The EMT phenotypes of SC were simple, with complete phenotype being the predominant form, and the morphological changes of the SCs were also relevant in terms of EMT. CONCLUSIONS: SC seems to be an irreversible, permanent change in the EMT phenomenon, with complete EMT phenotypes and various EMT-related pathways being involved in SC.
Chang Ohk Sung; Hannah Choi; Keun-Woo Lee; Seok-Hyung Kim
Related Documents :
16401477 - Reg iv activates the epidermal growth factor receptor/akt/ap-1 signaling pathway in col...
15533597 - Expression of glutathione s-transferase p1-1 in leukemic cells is regulated by inducibl...
9973257 - Ligand-activated retinoic acid receptor inhibits ap-1 transactivation by disrupting c-j...
8035787 - Regulation of c-jun expression during hypoxic and low-glucose stress.
21609737 - Temporally distinct expression of vesicular glutamate transporters 1 and 2 during embry...
22002997 - Gcn5 loss-of-function accelerates cerebellar and retinal degeneration in a sca7 mouse m...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-26
Journal Detail:
Title:  Journal of clinical pathology     Volume:  -     ISSN:  1472-4146     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Positive end-expiratory pressure aggravates left ventricular diastolic relaxation further in patient...
Next Document:  Myoepithelial and epithelial-myoepithelial, mesenchymal and fibroepithelial breast lesions: updates ...