Document Detail


Sarcoma cells induce alteration in adipogenic differentiation.
MedLine Citation:
PMID:  22493346     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Sarcomas consist of both tumor and stromal cells, and the interaction between these compartments is critical for tumor progression and metastasis. Tumor-derived factors may alter the differentiation capacity of the adjacent stromal cells. The aim of this study was to elucidate the paracrine impact of liposarcoma cells on pre-adipocytes, their adipogenic differentiation process and miRNA expression profile.
MATERIALS AND METHODS: 3T3-L1 pre-adipocytes were exposed to sarcoma pre-conditioned media. Following induction of adipogenic differentiation morphometrical changes were assessed. Differences in miRNA expression of conditioned and non-conditioned 3T3-L1 cells were analyzed.
RESULTS: Exposure to sarcoma pre-conditioned media substantially altered the differentiation capability of 3T3-L1 pre-adipocytes. Significant changes in the miRNA expression profile between conditioned and non-conditioned pre-adipocytes were observed.
CONCLUSION: Sarcoma cells directly modulate pre-adipocyte differentiation. This study demonstrates that sarcoma cells influence differentiation of pre-adipocytes via paracrine factors and alter their miRNA expression.
Authors:
Daniel J Tilkorn; Sammy Al-Benna; Joerg Hauser; Andrej Ring; Hans Ulrich Steinau; Andrea Tannapfel; Ingo Stricker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1167-73     Citation Subset:  IM    
Affiliation:
Department of Plastic Surgery, Reference Centre for Soft Tissue Sarcoma, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bürkle-de- la-Camp-Platz 1, 44789 Bochum, Germany. D.Tilkorn@web.de.
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