| Sarcolemmal and mitochondrial adenosine triphosphate- dependent potassium channels: mechanism of desflurane-induced cardioprotection. | |
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MedLine Citation:
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PMID: 10839925 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Volatile anesthetic-induced preconditioning is mediated by adenosine triphosphate-dependent potassium (KATP) channels; however, the subcellular location of these channels is unknown. The authors tested the hypothesis that desflurane reduces experimental myocardial infarct size by activation of specific sarcolemmal and mitochondrial KATP channels. METHODS: Barbiturate-anesthetized dogs (n = 88) were acutely instrumented for measurement of aortic and left ventricular pressures. All dogs were subjected to a 60-min left anterior descending coronary artery occlusion followed by 3-h reperfusion. In four separate groups, dogs received vehicle (0.9% saline) or the nonselective KATP channel antagonist glyburide (0.1 mg/kg intravenously) in the presence or absence of 1 minimum alveolar concentration desflurane. In four additional groups, dogs received 45-min intracoronary infusions of the selective sarcolemmal (HMR 1098; 1 microg. kg-1. min-1) or mitochondrial (5-hydroxydecanoate [5-HD]; 150 microg. kg-1. min-1) KATP channel antagonists in the presence or absence of desflurane. Myocardial perfusion and infarct size were measured with radioactive microspheres and triphenyltetrazolium staining, respectively. RESULTS: Desflurane significantly (P < 0.05) decreased infarct size to 10 +/- 2% (mean +/- SEM) of the area at risk as compared with control experiments (25 +/- 3% of area at risk). This beneficial effect of desflurane was abolished by glyburide (25 +/- 2% of area at risk). Glyburide (24 +/- 2%), HMR 1098 (21 +/- 4%), and 5-HD (24 +/- 2% of area at risk) alone had no effects on myocardial infarct size. HMR 1098 and 5-HD abolished the protective effects of desflurane (19 +/- 3% and 22 +/- 2% of area at risk, respectively). CONCLUSION: Desflurane reduces myocardial infarct size in vivo, and the results further suggest that both sarcolemmal and mitochondrial KATP channels could be involved. |
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Authors:
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W G Toller; E R Gross; J R Kersten; P S Pagel; G J Gross; D C Warltier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Anesthesiology Volume: 92 ISSN: 0003-3022 ISO Abbreviation: Anesthesiology Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-07-11 Completed Date: 2000-07-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1731-9 Citation Subset: AIM; IM |
Affiliation:
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Departments of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism,
physiology* Anesthetics, Inhalation / antagonists & inhibitors, pharmacology* Animals Benzamides / pharmacology Decanoic Acids / pharmacology Dogs Glyburide / pharmacology Hemodynamics / drug effects Hydroxy Acids / pharmacology Hypoglycemic Agents / pharmacology Ischemic Preconditioning, Myocardial Isoflurane / analogs & derivatives*, antagonists & inhibitors, pharmacology Mitochondria / metabolism* Myocardial Infarction / pathology, prevention & control Myocardium / pathology Potassium Channel Blockers Potassium Channels / agonists, metabolism* Regional Blood Flow Sarcolemma / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL03690/HL/NHLBI NIH HHS; HL54280/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics, Inhalation; 0/Benzamides; 0/Decanoic Acids; 0/HMR 1098; 0/Hydroxy Acids; 0/Hypoglycemic Agents; 0/Potassium Channel Blockers; 0/Potassium Channels; 10238-21-8/Glyburide; 26675-46-7/Isoflurane; 56-65-5/Adenosine Triphosphate; 57041-67-5/desflurane; 624-00-0/5-hydroxydecanoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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