| Saponins from the traditional medicinal plant Momordica charantia stimulate insulin secretion in vitro. | |
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MedLine Citation:
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PMID: 22133295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The antidiabetic activity of Momordica charantia (L.), Cucurbitaceae, a widely-used treatment for diabetes in a number of traditional medicine systems, was investigated in vitro. Antidiabetic activity has been reported for certain saponins isolated from M. charantia. In this study insulin secretion was measured in MIN6 β-cells incubated with an ethanol extract, saponin-rich fraction, and five purified saponins and cucurbitane triterpenoids from M. charantia, 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al (1), momordicine I (2), momordicine II (3), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O-β-glucopyranoside (4), and kuguaglycoside G (5). Treatments were compared to incubation with high glucose (27 mM) and the insulin secretagogue, glipizide (50 μM). At 125 μg/ml, an LC-ToF-MS characterized saponin-rich fraction stimulated insulin secretion significantly more than the DMSO vehicle, p=0.02. At concentrations 10 and 25 μg/ml, compounds 3 and 5 also significantly stimulated insulin secretion as compared to the vehicle, p≤0.007, and p=0.002, respectively. This is the first report of a saponin-rich fraction, and isolated compounds from M. charantia, stimulating insulin secretion in an in vitro, static incubation assay. |
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Authors:
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Amy C Keller; Jun Ma; Adam Kavalier; Kan He; Anne-Marie B Brillantes; Edward J Kennelly |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-11-30 |
Journal Detail:
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Title: Phytomedicine : international journal of phytotherapy and phytopharmacology Volume: 19 ISSN: 1618-095X ISO Abbreviation: Phytomedicine Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-05 Completed Date: 2012-06-05 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 9438794 Medline TA: Phytomedicine Country: Germany |
Other Details:
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Languages: eng Pagination: 32-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier GmbH. All rights reserved. |
Affiliation:
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Lehman College and the Graduate Center, City University of New York, Bronx, NY 10468, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / drug effects Cell Line Fruit / chemistry Hypoglycemic Agents / pharmacology Insulin / biosynthesis, secretion* Insulin-Secreting Cells / drug effects*, secretion* Mice Momordica charantia / chemistry* Phytotherapy Plant Extracts / pharmacology Plants, Medicinal / chemistry Saponins / isolation & purification, pharmacology* Seeds / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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F31 AT004548/AT/NCCAM NIH HHS; F31-AT004548/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Hypoglycemic Agents; 0/Insulin; 0/Plant Extracts; 0/Saponins |
| Comments/Corrections | |
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