Document Detail


Sanggenon C decreases tumor cell viability associated with proteasome inhibition.
MedLine Citation:
PMID:  21622138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several flavonoids have been reported to be proteasome inhibitors, but whether prenylated flavonoids are able to inhibit proteasome function remains unknown. We report for the first time that Sanggenon C, a natural prenylated flavonoid, inhibits tumor cellular proteasomal activity and cell viability. We found that (1) Sanggenon C inhibited tumor cell viability and induced cell cycle arrest at G0/G1 phase; (2) Sanggenon C inhibited the chymotrypsin-like activity of purified human 20S proteasome and 26S proteasome in H22 cell lysate, and Sanggenon C was able to dose-dependently accumulate ubiquitinated proteins and proteasome substrate protein p27; (3) Sanggenon C-induced proteasome inhibition occurred prior to cell death in murine H22 and P388 cell lines; (4) Sanggenon C induced death of human K562 cancer cells and primary cells isolated from leukemic patients. We conclude that Sanggenon C inhibits tumor cell viability via induction of cell cycle arrest and cell death, which is associated with its ability to inhibit the proteasome function and that proteasome inhibition by Sanggenon C at least partially contributes to the observed tumor cell growth-inhibitory activity.
Authors:
Hongbiao Huang; Ningning Liu; Kai Zhao; Chenchen Zhu; Xiaoyu Lu; Shujue Li; Wen Lian; Ping Zhou; Xiaoxian Dong; Canguo Zhao; Haiping Guo; Change Zhang; Changshan Yang; Guanmei Wen; Li Lu; Xiaofen Li; Lixia Guan; Chunjiao Liu; Xuejun Wang; Qing Ping Dou; Jinbao Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  3     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-30     Completed Date:  2011-10-10     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1315-25     Citation Subset:  IM    
Affiliation:
Department of Pathophysiology, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou Guangzhou Medical College, Guangzhou, Guangdong, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzofurans / pharmacology*
Blotting, Western
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Chromones / pharmacology*
Mice
Grant Support
ID/Acronym/Agency:
1R01CA120009/CA/NCI NIH HHS; 3R01CA120009-04S1/CA/NCI NIH HHS; R01 CA120009-04/CA/NCI NIH HHS; R01 CA120009-04S1/CA/NCI NIH HHS; R01 CA120009-05/CA/NCI NIH HHS; R01 HL072166-07/HL/NHLBI NIH HHS; R01 HL072166-08/HL/NHLBI NIH HHS; R01 HL072166-09/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Benzofurans; 0/Chromones; 80651-76-9/sanggenone C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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