Document Detail


Sandwich enzyme-linked immunosorbent assay (ELISA) for detection of mustard in foods.
MedLine Citation:
PMID:  18460147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Undeclared mustard residues in food products could trigger allergic reactions in mustard-allergic consumers. Our objective was to develop and validate a sandwich-type ELISA for the detection of mustard residues in foods. A mixture of yellow, brown, and oriental mustard seeds was used to immunize 3 rabbits and 1 sheep. Two mustard ELISAs were developed by utilizing the reciprocal combination of rabbit and sheep polyclonal antimustard sera as the capture and detector reagents. Binding was visualized by addition of rabbit antisheep or goat antirabbit IgG antibody labeled with alkaline phosphatase and subsequent addition of substrate. The optimized ELISAs have limits of quantification (LOQ) of 1 and 3 ppm (mug of ground, whole mustard seeds/mL) for the sheep capture and rabbit capture formats, respectively. Only rapeseed cross-reacted in the rabbit and sheep capture mustard ELISAs at a level equivalent to 12300 and 16900 ppm of mustard. The mean percent recovery for cooked frankfurters spiked with 0 to 1000 ppm mustard flour was 95.3%+/- 10.7%. A limited retail survey of 29 foods revealed that, of 15 samples having mustard declared on the ingredient list, 2 baked bean products contained no detectable mustard, possibly owing to a decrease in extractability and detectability of mustard proteins after subjecting to thermal processing. For the remaining 14 samples without mustard declared on the label, 3 samples contained detectable mustard, presumably due to the labeling of mustard as "spice" or inadvertent cross-contamination. This sandwich-type ELISA can serve as a powerful tool for food manufacturers and regulatory agencies to detect and quantify mustard residues in processed foods.
Authors:
P-W Lee; S L Hefle; S L Taylor
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food science     Volume:  73     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-07     Completed Date:  2008-06-16     Revised Date:  2009-01-29    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  T62-8     Citation Subset:  IM    
Affiliation:
Food Allergy Research and Resource Program, Univ. of Nebraska, 143 Food Industry Complex, Lincoln, NE 68583-0919, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis
Animals
Antibody Specificity
Enzyme-Linked Immunosorbent Assay / methods*
Food Analysis*
Food Hypersensitivity / prevention & control
Immune Sera / biosynthesis,  immunology
Immunization
Indicators and Reagents
Meat Products / analysis
Mustard Plant / chemistry*,  immunology
Rabbits / immunology
Seeds / chemistry,  immunology
Sheep / immunology
Chemical
Reg. No./Substance:
0/Allergens; 0/Immune Sera; 0/Indicators and Reagents
Comments/Corrections
Comment In:
J Food Sci. 2008 Oct;73(8):viii   [PMID:  19019138 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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