Document Detail


"Sandwich" enzyme immunoassay for serum ferritin with polypropylene test tubes as the solid phase.
MedLine Citation:
PMID:  6403260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A "sandwich" enzyme immunoassay for ferritin in human serum is described, in which horseradish peroxidase-labeled antibody and a very sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazolin-6-sulfonate), are used. The solid phase is polypropylene test tubes treated with glutaraldehyde; immunologic reactions are developed during constant rotation of the tubes. The assay requires 20 microL of serum per assay and takes less than 3 h. The standard curve is linear between 0 and 400 micrograms of serum ferritin per liter. Analytical recoveries of various amounts of standard added to a serum sample ranged from 92 to 98.6%. The sensitivity is 2 micrograms/L, which translates to 0.4 ng per assay tube. Within-run CV ranges from 1.6 to 5.4% and between-run from 4.2 to 7.8% according to the ferritin concentration. Results by competitive radioimmunoassay correlated well (r = 0.98). No "hook effect" was observed for concentrations up to 13 mg/L. The reference interval is 40-240 micrograms/L for men, 8-100 micrograms/L for women.
Authors:
M C Revenant
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  681-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromogenic Compounds
Ferritins / blood*
Glutaral
Horseradish Peroxidase
Humans
Immunoenzyme Techniques
Plastics*
Polypropylenes*
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Plastics; 0/Polypropylenes; 111-30-8/Glutaral; 9007-73-2/Ferritins; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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