| Sampling of prenatal and postnatal offspring from individual rat dams enhances animal use without compromising development. | |
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MedLine Citation:
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PMID: 11542953 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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To assess prenatal and postnatal developmental status in the offspring of a group of animals, it is typical to examine fetuses from some of the dams as well as infants born to the remaining dams. Statistical limitations often arise, particularly when the animals are rare or especially precious, because all offspring of the dam represent only a single statistical observation; littermates are not independent observations (biologically or statistically). We describe a study in which pregnant laboratory rats were laparotomized on day 7 of gestation (GD7) to ascertain the number and distribution of uterine implantation sites and were subjected to a simulated experience on a 10-day space shuttle flight. After the simulated landing on GD18, rats were unilaterally hysterectomized, thus providing a sample of fetuses from 10 independent uteruses, followed by successful vaginal delivery on GD22, yielding postnatal samples from 10 uteruses. A broad profile of maternal and offspring morphologic and physiologic measures indicated that these novel sampling procedures did not compromise maternal well-being and maintained normal offspring development and function. Measures included maternal organ weights and hormone concentrations, offspring body size, growth, organ weights, sexual differentiation, and catecholamine concentrations. |
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Authors:
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J R Alberts; H W Burden; N Hawes; A E Ronca |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science Volume: 35 ISSN: 1060-0558 ISO Abbreviation: Contemp Top Lab Anim Sci Publication Date: 1996 Nov |
Date Detail:
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Created Date: 2000-05-28 Completed Date: 2000-05-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9204153 Medline TA: Contemp Top Lab Anim Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 61-5 Citation Subset: S |
Affiliation:
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Indiana University, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands Animals Animals, Newborn / growth & development* Body Weight Embryonic and Fetal Development* Evaluation Studies as Topic Feasibility Studies Female Hysterectomy Laparotomy Male Organ Size Pregnancy Rats Rats, Sprague-Dawley / embryology* Research Design Thymus Gland Uterus / surgery Weightlessness Simulation* |
| Investigator | |
Investigator/Affiliation:
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J R Alberts / IN U, Bloomington; H W Burden / E Carolina U, Greenville, NC |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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