Document Detail

Sampling of prenatal and postnatal offspring from individual rat dams enhances animal use without compromising development.
MedLine Citation:
PMID:  11542953     Owner:  NASA     Status:  MEDLINE    
To assess prenatal and postnatal developmental status in the offspring of a group of animals, it is typical to examine fetuses from some of the dams as well as infants born to the remaining dams. Statistical limitations often arise, particularly when the animals are rare or especially precious, because all offspring of the dam represent only a single statistical observation; littermates are not independent observations (biologically or statistically). We describe a study in which pregnant laboratory rats were laparotomized on day 7 of gestation (GD7) to ascertain the number and distribution of uterine implantation sites and were subjected to a simulated experience on a 10-day space shuttle flight. After the simulated landing on GD18, rats were unilaterally hysterectomized, thus providing a sample of fetuses from 10 independent uteruses, followed by successful vaginal delivery on GD22, yielding postnatal samples from 10 uteruses. A broad profile of maternal and offspring morphologic and physiologic measures indicated that these novel sampling procedures did not compromise maternal well-being and maintained normal offspring development and function. Measures included maternal organ weights and hormone concentrations, offspring body size, growth, organ weights, sexual differentiation, and catecholamine concentrations.
J R Alberts; H W Burden; N Hawes; A E Ronca
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science     Volume:  35     ISSN:  1060-0558     ISO Abbreviation:  Contemp Top Lab Anim Sci     Publication Date:  1996 Nov 
Date Detail:
Created Date:  2000-05-28     Completed Date:  2000-05-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9204153     Medline TA:  Contemp Top Lab Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-5     Citation Subset:  S    
Indiana University, USA.
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MeSH Terms
Adrenal Glands
Animals, Newborn / growth & development*
Body Weight
Embryonic and Fetal Development*
Evaluation Studies as Topic
Feasibility Studies
Organ Size
Rats, Sprague-Dawley / embryology*
Research Design
Thymus Gland
Uterus / surgery
Weightlessness Simulation*
J R Alberts / IN U, Bloomington; H W Burden / E Carolina U, Greenville, NC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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