Document Detail


Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp.
MedLine Citation:
PMID:  18276190     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From comparative analysis of EST data for five taxa within the eukaryotic supergroup Amoebozoa, including two free-living amoebae (Acanthamoeba castellanii, Hartmannella vermiformis) and three slime molds (Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp.), we obtained new broad-range perspectives on the evolution and biosynthetic capacity of this assemblage. Together with genome sequences for the amoebozoans Dictyostelium discoideum and Entamoeba histolytica, and including partial genome sequence available for A. castellanii, we used the EST data to identify genes that appear to be exclusive to the supergroup, and to specific clades therein. Many of these genes are likely involved in cell-cell communication or differentiation. In examining on a broad scale a number of characters that previously have been considered in simpler cross-species comparisons, typically between Dictyostelium and Entamoeba, we find that Amoebozoa as a whole exhibits striking variation in the number and distribution of biosynthetic pathways, for example, ones for certain critical stress-response molecules, including trehalose and mannitol. Finally, we report additional compelling cases of lateral gene transfer within Amoebozoa, further emphasizing that although this process has influenced genome evolution in all examined amoebozoan taxa, it has done so to a variable extent.
Authors:
Russell F Watkins; Michael W Gray
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-13
Journal Detail:
Title:  Protist     Volume:  159     ISSN:  1434-4610     ISO Abbreviation:  Protist     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806488     Medline TA:  Protist     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  269-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5.
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MeSH Terms
Descriptor/Qualifier:
Acanthamoeba castellanii / genetics
Amoebida / classification,  genetics*,  physiology
Animals
Biodiversity*
Carbohydrate Metabolism
DNA, Protozoan / genetics
Evolution, Molecular
Expressed Sequence Tags*
Gene Library
Gene Transfer, Horizontal
Genes, Protozoan*
Genome, Protozoan
Hartmannella / genetics
Meiosis
Physarum polycephalum / classification,  genetics*,  physiology
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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