Document Detail


Sample purification on a microfluidic device.
MedLine Citation:
PMID:  11700715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sample preparation has long been recognized as a significant barrier to the implementation of macroscopic protocols on microfabricated devices. Macroscopically, such tasks as removing salts, primers and other contaminants are performed by methods involving precipitation, specialized membranes and centrifuges, none of which are readily performed in microfluidic structures. Although some microfluidic systems have been developed for performing sample purification, their complexity may hinder the degree to which they can be implemented. We present a method of microchip-based sample purification that can be performed with even the simplest microfluidic designs. The technique is demonstrated by removing primers from a sample of amplified DNA, leaving only the product DNA. This provides a new sample preparation capability for microfluidic systems.
Authors:
T Footz; S Wunsam; S Kulak; H J Crabtree; D M Glerum; C J Backhouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  22     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-11-09     Completed Date:  2002-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3868-75     Citation Subset:  IM    
Affiliation:
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.
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MeSH Terms
Descriptor/Qualifier:
DNA / isolation & purification*
DNA Primers
Electrophoresis, Capillary / instrumentation*
Fluorometry / instrumentation
Microchemistry / instrumentation*
Microscopy, Confocal / instrumentation
Polymerase Chain Reaction / instrumentation
Rheology*
Chemical
Reg. No./Substance:
0/DNA Primers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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