| Sample processing and methodological pitfalls in multiple myeloma research. | |
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MedLine Citation:
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PMID: 21923059 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In this paper, initial processing of biological material, cell separation algorithms and other procedures are discussed. For samples with initial infiltration of plasma cells > 5%, CD138 MicroBeads and Auto-Magnetic-Activated Cell Sorting program are used. Fluorescence-Activated Cell Sorting is used exclusively for cell populations with low-abundance; these samples are detected using fluorescently labeled antibodies only. Isolated plasma cells are further processed for molecular biological studies, for cytogenetics and protein analyses. Furthermore, this work examines the pitfalls of research related to multiple myeloma; some of them we have overcome, while the others are still problematic. |
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Authors:
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A Potácová; J Stossová; I Buresová; L Kovárová; M Almási; M Penka; R Hájek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Klinická onkologie : casopis Ceské a Slovenské onkologické spolecnosti Volume: 24 Suppl ISSN: 0862-495X ISO Abbreviation: Klin Onkol Publication Date: 2011 |
Date Detail:
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Created Date: 2011-09-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9425213 Medline TA: Klin Onkol Country: Czech Republic |
Other Details:
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Languages: eng Pagination: S18-23 Citation Subset: IM |
Affiliation:
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Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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