| Sample preparation for amino acid determination by integrated pulsed amperometric detection in foods. | |
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MedLine Citation:
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PMID: 12423635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This report describes a new sample preparation method for food which allows a complete separation of carbohydrates and amino acids prior to their analysis by anion-exchange chromatography and integrated pulsed amperometric detection. Food samples with high carbohydrate concentrations are applied to solid-phase extraction columns containing a strong cation-exchange resin. Carbohydrates are recovered initially; retained amino acids are eluted with 0.2 M CaC l(2) subsequently. The carbohydrate and the amino acid fractions are analyzed. The recovery calculated for 21 amino acids was in the range from 84 to 126%. The sample preparation was tested for amino acid concentrations between 4.2 and 84.0 nmol of each amino acid (between 2.1 and 42.0 nmol of cystine) and correlation coefficients between 0.84 and 0.99 were obtained. The capacity of the solid-phase extraction columns employed was up to 3.7 micro mol. Sample preparation was evaluated with four different food samples: sourdough, skim milk, lemon juice, and potato. |
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Authors:
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C Thiele; M G Gänzle; R F Vogel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 310 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-11-08 Completed Date: 2003-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 171-8 Citation Subset: IM |
Affiliation:
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TU-München-Weihenstephan, Lehrstuhl für Technische Mikrobiologie, D-85350, Freising, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Amino Acids / analysis*, isolation & purification Calcium Chloride / chemistry Carbohydrates / analysis*, isolation & purification Cation Exchange Resins Chemistry, Physical / methods* Chromatography, High Pressure Liquid / methods Chromatography, Ion Exchange / methods Electrochemistry / methods Food Analysis / methods* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Cation Exchange Resins; 10043-52-4/Calcium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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