Document Detail


Sample preparation for amino acid determination by integrated pulsed amperometric detection in foods.
MedLine Citation:
PMID:  12423635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report describes a new sample preparation method for food which allows a complete separation of carbohydrates and amino acids prior to their analysis by anion-exchange chromatography and integrated pulsed amperometric detection. Food samples with high carbohydrate concentrations are applied to solid-phase extraction columns containing a strong cation-exchange resin. Carbohydrates are recovered initially; retained amino acids are eluted with 0.2 M CaC l(2) subsequently. The carbohydrate and the amino acid fractions are analyzed. The recovery calculated for 21 amino acids was in the range from 84 to 126%. The sample preparation was tested for amino acid concentrations between 4.2 and 84.0 nmol of each amino acid (between 2.1 and 42.0 nmol of cystine) and correlation coefficients between 0.84 and 0.99 were obtained. The capacity of the solid-phase extraction columns employed was up to 3.7 micro mol. Sample preparation was evaluated with four different food samples: sourdough, skim milk, lemon juice, and potato.
Authors:
C Thiele; M G Gänzle; R F Vogel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  310     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-08     Completed Date:  2003-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  171-8     Citation Subset:  IM    
Affiliation:
TU-München-Weihenstephan, Lehrstuhl für Technische Mikrobiologie, D-85350, Freising, Germany.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Amino Acids / analysis*,  isolation & purification
Calcium Chloride / chemistry
Carbohydrates / analysis*,  isolation & purification
Cation Exchange Resins
Chemistry, Physical / methods*
Chromatography, High Pressure Liquid / methods
Chromatography, Ion Exchange / methods
Electrochemistry / methods
Food Analysis / methods*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Cation Exchange Resins; 10043-52-4/Calcium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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