Document Detail


Sample preparation induced artifacts in cryo-electron tomographs.
MedLine Citation:
PMID:  23040048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effects of sample preparation and of the exposure to an electron beam on particles in cryo-electron tomographs. Various virus particles with icosahedral symmetry were examined, allowing a comparison of symmetrically related components that should be identical in structure but might be affected differently by these imaging artifacts. Comparison of tomographic reconstructions with previously determined structures established by an independent method showed that neither freezing nor electron beam exposure produced a significant amount of shrinkage along the z axis (thickness). However, we observed damage to regions of the particles located close to the surface of the vitreous ice.
Authors:
Pavel Plevka; Anthony J Battisti; Dennis C Winkler; Kaspars Tars; Heather A Holdaway; Carol M Bator; Michael G Rossmann
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2012-10-08
Journal Detail:
Title:  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada     Volume:  18     ISSN:  1435-8115     ISO Abbreviation:  Microsc. Microanal.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-25     Completed Date:  2013-03-22     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  9712707     Medline TA:  Microsc Microanal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1043-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907-2032, USA.
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MeSH Terms
Descriptor/Qualifier:
Artifacts
Bacteriophages / chemistry,  ultrastructure*
Cryoelectron Microscopy / methods*
Cytological Techniques / methods*
Viruses / chemistry,  ultrastructure*
Grant Support
ID/Acronym/Agency:
AI 76331/AI/NIAID NIH HHS; R01 AI076331/AI/NIAID NIH HHS; R37 AI011219/AI/NIAID NIH HHS
Comments/Corrections

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