Document Detail

Salvianolic acid a suppress lipopolysaccharide-induced NF-κB signaling pathway by targeting IKKβ
MedLine Citation:
PMID:  21839184     Owner:  NLM     Status:  Publisher    
Salvianolic acid A, an active compound present in Salvia miltiorrhiza, is a phenolic carboxylic acid derivative, ((2R)-3-(3,4-Dihydroxyphenyl)-2-[(E)-3-[2-[(E)-2-(3,4-dihydroxyphenyl) ethenyl]-3,4-dihydroxyphenyl] prop-2-enoyl]oxypropanoic acid). The present study was performed to investigate the underlying mechanisms of anti-inflammatory effects with salvianolic acid A, specially focused on nuclear factor κB (NF-κB) signaling pathway by targeting the IκB kinase β (IKKβ). The effect of salvianolic acid A for IKKβ activity was analyzed using an immobilized metal affinity for phosphochemicals (IMAP)-based time-resolved fluorescence resonance energy transfer (TR-FRET) assay. The underlying mechanisms of salvianolic acid A were examined using lipopolysaccharide (LPS)-stimulated RAW264.7 cells. IKKβ studies based on IMAP-TR-FRET showed that salvianolic acid A possesses a potent IKKβ inhibitory activity with Ki value of 3.63μM in an ATP-noncompetitive manner. Pretreatment with salvianolic acid A (10, 30μM) decreased LPS-induced expression of iNOS and COX-2, thereby inhibiting production of nitric oxide and prostaglandin E(2), respectively. In addition, salvianolic acid A (10, 30μM) also attenuated the LPS-induced IκBα phosphorylation and degradation and NF-κB translocation. These results suggest that salvianolic acid A modulates NF-κB-dependent inflammatory pathways through IKKβ inhibition and these anti-inflammatory effects will aid in understanding the pharmacology and mode of action of salvianolic acid A.
Kwang-Seok Oh; Byung Koo Oh; Jihye Mun; Ho Won Seo; Byung Ho Lee
Related Documents :
7673064 - Prenatal androgenization of lambs: ii. metabolism in adipose tissue and liver.
23381564 - Mechanism of arylboronic acid-catalyzed amidation reaction between carboxylic acids and...
2161664 - New technique for processing paraffin-embedded tissue for y chromosome fluorescence ide...
16658264 - Polar indole-3-acetic acid diffusion in nonliving and model systems.
22349344 - Accurate and reliable quantification of total microalgal fuel potential as fatty acid m...
8508634 - The allergenicity of glycerol esters and other esters of rosin (colophony).
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-9
Journal Detail:
Title:  International immunopharmacology     Volume:  -     ISSN:  1878-1705     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965259     Medline TA:  Int Immunopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Finding a better path to drug selectivity.
Next Document:  Hippocampal cell response to substrates with surface chemistry gradients.