| Salvianolic acid B suppresses human monocyte-derived dendritic cells maturation via PPAR-γ activation. | |
| | |
MedLine Citation:
|
PMID: 21649636 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background and purpose: Salvianolic acid B (Sal B), a water-soluble antioxidant derived from a Chinese medicinal herb, is known to be effective in the prevention of atherosclerosis. Here, we tested the hypothesis that the anti-atherosclerotic effect of Sal B might be mediated by suppressing human monocyte-derived dendritic cells (h-monDC) maturation. Experimental approach: H-monDC was derived by incubating purified human monocytes with GM-CSF and IL-4. H-monDC was pre-incubated with or without Sal B and stimulated by oxidized low density lipoprotein (ox-LDL) in the presence or absence of PPAR-γ siRNA. Expression of h-monDC membrane molecules (CD40, CD86, CD1a, HLA-DR) were analyzed by FACS, cytokines were measured by ELISA, and TLR4 associated signaling pathway was determined by Western blotting. Key results: Ox-LDL promoted h-monDC maturation, stimulated CD40, CD86, CD1a, HLA-DR expression and IL-12, IL-10, TNF-α production and up-regulated TLR4 signaling. These effects could be significantly inhibited by Sal B. Sal B also triggered PPAR-γ activation and promoted PPAR-γ nuclear translocation, attenuated ox-LDL-induced up-regulation of TLR4 and myeloid differentiation primary-response protein 88 (MyD88), and inhibited downstream p38-MAPK signaling cascade. Knocking down PPAR-γ through siRNA transfection significantly blocked above mentioned effects of Sal B. Conclusions and implications: Our data suggested that Sal B effectively suppressed ox-LDL-induced h-monDC maturation through PPAR-γ activation. |
| | |
Authors:
|
Aijun Sun; Hongying Liu; Shijun Wang; Dazhuo Shi; Lei Xu; Yong Cheng; Keqiang Wang; Keji Chen; Yunzeng Zou; Junbo Ge |
Related Documents
:
|
20127046 - Amelioration of asthmatic inflammation by an aqueous extract of spinacia oleracea linn. 20817776 - Human mesenchymal stem cells suppress chronic airway inflammation in the murine ovalbum... 11503516 - Pathophysiological mechanisms of asthma. application of cell and molecular biology tech... 8625546 - Intravenous immune globulin: an alternative therapy in steroid-dependent allergic disea... 8557986 - Herpes simplex virus type 1-mediated up-regulation of il-12 (p40) mrna expression. impl... 20674676 - Protective effect of (-)clausenamide against abeta-induced neurotoxicity in differentia... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-7 |
Journal Detail:
|
Title: British journal of pharmacology Volume: - ISSN: 1476-5381 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-8 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2011 The Authors. British Journal of Pharmacology © 2011 The British Pharmacological Society. |
Affiliation:
|
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR China; Institute of Biomedical Science, Fudan University, Shanghai 200032, PR China; and China Academy of Chinese Medical Sciences, Xiyuan Hospital, Beijing 100091, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Predictors of extraordinary survival in the Iowa established populations for epidemiologic study of ...
Next Document: CANNABINOIDS AND BONE: ENDOCANNABINOIDS MODULATE HUMAN OSTEOCLAST FUNCTION IN VITRO.