Document Detail


Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry.
MedLine Citation:
PMID:  25182726     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H2O2-induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa, native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases.
METHODS: Dried leaves of S. fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations. Each mL of the preparation contained (7.1±1.0) mg of extract. HEK-293 cells were incubated in one set with S. fruticosa extract in the presence of 0.1 mmol/L H2O2, and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivatization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique.
RESULTS: Cells incubated 3 h with 150 µL extract and exposed to 0.1 mmol/L H2O2 showed lower intensity of fluorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 µL of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S. fruticosa).
CONCLUSIONS: The results from this study suggest that the water-soluble extract of S. fruticosa leaves protects against both H2O2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.
Authors:
Saleem Bani Hani; Mekki Bayachou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical biomedicine     Volume:  4     ISSN:  2221-1691     ISO Abbreviation:  Asian Pac J Trop Biomed     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-09-03     Completed Date:  2014-09-03     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  101557706     Medline TA:  Asian Pac J Trop Biomed     Country:  China    
Other Details:
Languages:  eng     Pagination:  399-403     Citation Subset:  -    
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