Document Detail

Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry.
MedLine Citation:
PMID:  25182726     Owner:  NLM     Status:  PubMed-not-MEDLINE    
OBJECTIVE: To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H2O2-induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa, native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases.
METHODS: Dried leaves of S. fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations. Each mL of the preparation contained (7.1±1.0) mg of extract. HEK-293 cells were incubated in one set with S. fruticosa extract in the presence of 0.1 mmol/L H2O2, and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivatization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique.
RESULTS: Cells incubated 3 h with 150 µL extract and exposed to 0.1 mmol/L H2O2 showed lower intensity of fluorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 µL of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S. fruticosa).
CONCLUSIONS: The results from this study suggest that the water-soluble extract of S. fruticosa leaves protects against both H2O2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.
Saleem Bani Hani; Mekki Bayachou
Related Documents :
3287616 - Dna damage and oxygen radical toxicity.
11448456 - Involvement of nadph: cytochrome p450 reductase in the activation of indoloquinone eo9 ...
9776756 - Guanine is the target for direct ionisation damage in dna, as detected using excision e...
10857906 - Zinc tetraruthenated porphyrin binding and photoinduced oxidation of calf-thymus dna.
20010696 - Transduction of rna-directed dna methylation signals to repressive histone marks in ara...
2111856 - Dna/dna hybridization studies of the carnivorous marsupials. i: the intergeneric relati...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical biomedicine     Volume:  4     ISSN:  2221-1691     ISO Abbreviation:  Asian Pac J Trop Biomed     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-09-03     Completed Date:  2014-09-03     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  101557706     Medline TA:  Asian Pac J Trop Biomed     Country:  China    
Other Details:
Languages:  eng     Pagination:  399-403     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae).
Next Document:  Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison...