| Salt-induced lipid changes in Catharanthus roseus cultured cell suspensions. | |
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MedLine Citation:
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PMID: 15279997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salt treatment strongly affected cell growth by decreasing dry weight. Exposure of Catharanthus roseus cell suspensions to increasing salinity significantly enhanced total lipid (TL) content. The observed increase is mainly due to high level of phospholipids (PL). Hundred mM NaCl treatment increased phospholipid species phosphatidylcholine (PC) and phosphatidylethanolamine (PE), whereas it reduced glycolipid ones monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) but not sulfoquinovosyldiacylglycerol (SQDG). Moreover, fatty acid composition was clearly modified when cells were cultured in the presence of 100 mM NaCl, whereas only few changes occurred at 50 mM. Salt treatment decreased palmitic acid (16:0) level and increased that of linolenic acid (18:2). Such effect was observed in phospholipid species PC and PE and in glycolipid DGDG. Double bond index (DBI) was enhanced more than 2-fold in fatty acids of either glycolipids or phospholipids from cells submitted to 100 mM NaCl. Free sterol content was also significantly enhanced, especially at 100 mM NaCl, whereas free sterols/phospholipids (St/PL) ratio was slightly decreased. All these salt-induced changes in membrane lipids suggest an increase in membrane fluidity of C. roseus cells. |
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Authors:
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Salem Elkahoui; Abderrazek Smaoui; Mokhtar Zarrouk; Rachid Ghrir; Férid Limam |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytochemistry Volume: 65 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-28 Completed Date: 2004-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 1911-7 Citation Subset: IM |
Affiliation:
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Laboratoire Interactions Légumineuses-Microorganismes, Institut National de Recherche Scientifique et Technique, BP 95-2050 Hammam-Lif, Tunisia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Catharanthus
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drug effects,
growth & development* Cells, Cultured Culture Media Fatty Acids / analysis Glycolipids / chemistry, metabolism Membrane Fluidity* Phospholipids / chemistry, metabolism Sodium Chloride / pharmacology* Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Glycolipids; 0/Phospholipids; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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