Document Detail


Salt-induced changes in lipid composition and membrane fluidity of halophilic yeast-like melanized fungi.
MedLine Citation:
PMID:  15064990     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The halophilic melanized yeast-like fungi Hortaea werneckii, Phaeotheca triangularis, and the halotolerant Aureobasidium pullulans, isolated from salterns as their natural environment, were grown at different NaCl concentrations and their membrane lipid composition and fluidity were examined. Among sterols, besides ergosterol, which was the predominant one, 23 additional sterols were identified. Their total content did not change consistently or significantly in response to raised NaCl concentrations in studied melanized fungi. The major phospholipid classes were phosphatidylcholine and phosphatidylethanolamine, followed by anionic phospholipids. The most abundant fatty acids in phospholipids contained C16 and C18 chain lengths with a high percentage of C18:2Delta9,12. Salt stress caused an increase in the fatty acid unsaturation in the halophilic H. werneckii and halotolerant A. pullulans but a slight decrease in halophilic P. triangularis. All the halophilic fungi maintained their sterol-to-phospholipid ratio at a significantly lower level than did the salt-sensitive Saccharomyces cerevisiae and halotolerant A. pullulans. Electron paramagnetic resonance (EPR) spectroscopy measurements showed that the membranes of all halophilic fungi were more fluid than those of the halotolerant A. pullulans and salt-sensitive S. cerevisiae, which is in good agreement with the lipid composition observed in this study.
Authors:
Martina Turk; Laurence Méjanelle; Marjeta Sentjurc; Joan O Grimalt; Nina Gunde-Cimerman; Ana Plemenitas
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-11-13
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  8     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-05     Completed Date:  2004-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  53-61     Citation Subset:  IM; S    
Affiliation:
Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Ascomycota / drug effects*,  isolation & purification,  metabolism*
Electron Spin Resonance Spectroscopy
Melanins / metabolism
Membrane Fluidity / drug effects
Membrane Lipids / metabolism*
Phospholipids / metabolism
Sodium Chloride / pharmacology*
Species Specificity
Sterols / metabolism
Chemical
Reg. No./Substance:
0/Melanins; 0/Membrane Lipids; 0/Phospholipids; 0/Sterols; 7647-14-5/Sodium Chloride

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